A production method for cryopreservation and regeneration of euonymus pollen

Abstract

The invention relates to an ultralow-temperature preservation method for pollen of euonymus bungeanus. Fresh euonymus bungeanus pollen is collected from a well-developed mother tree and then placed into 1.8-ml frozen preservation tube, the frozen preservation tube is then placed in a drying vessel containing a silica gel desiccant and dried for 10 hours to keep the pollen water content at 4%-4.5%; the frozen preservation tube with pollen is put into a liquid nitrogen tank at subzero 196 DEG C for preservation, and then defrosting is performed at the room temperature for 30 min; a culture solution formula adopted for germination of the defrosted pollen comprises sucrose with concentration of 100 g / L and boric acid with concentration of 150 mg / L. The method is easy to operate, safe and stable, occupied space is small, the preservation cost is low, the preservation time of pollen is greatly prolonged, long-term storage and exchange of pollen become possible, and the pollen after being preserved at the ultra-low temperature can preserve genetic integrity and stability. In addition, introduction of pests and diseases can also be avoided in a low-temperature environment, and the method has important practice and popularization significance in hybrid breeding and molecular research of the euonymus bungeanus.

Description

technical field

[0001] The invention relates to a production method for cryopreservation and regeneration of euonymus pollen, belonging to the fields of garden production and germplasm resource protection. Background technique

[0002] Euonymus peach leaf, also known as Baidu and Simianmu, is a small tree of the genus Euonymus in the family Euonymus. It is one of the common ornamental garden tree species in my country. It has beautiful leaves, luxuriant plants, and is covered with red arils. The fruit can be stored on the branches for a long time. In winter, the white snow and red fruit are very beautiful, adding a lot of color to the northern gardens in winter. Euonymus japonicus has good adaptability in cold tolerance, drought resistance, wind resistance, etc. Total saponins of Euonymus japonicus fruit have obvious growth inhibitory effect on human liver cancer cell line-SMMC, human cervical cancer cell line-HeLa and breast cancer cell line-MCF7 ; The seeds of Euonymus pea...

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