Kit for culturing cardiac progenitor cells

Abstract

The invention provides a kit for culturing cardiac progenitor cells. A plurality of reagent bottles are arranged in the kit and contain a culture solution I, a culture solution II, a growth factor I, a growth factor II, a growth factor III, a growth factor IV, a digestive solution, a cleaning solution, a cryopreservation solution and cell seeds respectively. By the adoption of the kit, culture, amplification and cryopreservation of cardiac progenitor cells can be achieved, the problems that the culture survival rate of cardiac progenitor cells is low, culture efficiency is low, inter-batch difference is large, and cryopreservation reactivation rate is not ideal are solved, cardiac progenitor cells can be amplified in an ideal nutrient balance state without differentiation, and rich sources are provided for further experimental study and medical treatment using cardiac progenitor cells. The kit is quite suitable for cardiac progenitor cell culture.

Description

technical field [0001] The invention belongs to the field of cell technology, in particular to a kit for cultivating myocardial progenitor cells. Background technique [0002] In recent years, the morbidity and mortality of myocardial infarction have been increasing year by year, and it is one of the important diseases that threaten human health today. Studies have found that after myocardial infarction, a small number of cardiomyocytes will divide and proliferate, but the number is limited, limited to the surviving part of the myocardium, and cannot effectively repair myocardial tissue; a large number of cardiomyocytes are replaced by scar tissue after death, resulting in local myocardial contraction weakening, Disturbance, myocardial function decline, there is no ideal treatment at present, how to increase the number of myocardial cells with systolic function becomes the key to the treatment of heart failure after myocardial damage. [0003] Cardiomyoplasty, the transplan...

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Problems solved by technology

[0004] In order to meet the needs of medical research and treatment, cultivating a large number of myocardial progenitor cells has become an important research topic. The process of culturing myocardial progenitor cells is very cumbersome, and there are many types of culture reagents required, and the culture time is long. If the experimenter chooses The reagents and methods used to culture and preserve myocardial progenitor cells are not appropriate, often resulting in the quantity and quality of the cultured myocardial progenitor cells failing to meet the requirements, wasting manpower and other resources, so a special method for stable large-scale expansion is studied. The kit for culturing cardiac progenitor cells is of great significance

At present, there are few kits specially used for culturing myocardial progenitor cells. Some kits for cultivating other stem cells are disclosed in the prior art. For example, CN104928238A discloses a kit for preparing adipose-derived mesenchymal stem cells, including adipose Preservation solution, fat washing solution, lipolysis solution, cell washing solution, cell culture solution, cell digestion solution, and cell cryopreservation solution, but the effect of this kit on culturing and expanding stem cells is not ideal, and the safety and stability of the reagents are not high , low cell viability and proliferation

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