Nucleic acid composition for detecting HPV (Human Papilloma Virus) types as well as application and kit thereof

A kit and nucleic acid technology, applied in the directions of microorganism-based methods, microorganism determination/inspection, biochemical equipment and methods, etc., can solve the problems of cumbersome operation methods, low sensitivity or specificity, etc. Sensitivity, effect of increasing quantity

Inactive Publication Date: 2017-05-31
北京易活生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is still a lack of effective treatment methods for HPV, so early detection and prevention of cervical HPV are the key to blocking canceration. However, the existing related technologies for HPV typing detection (including traditional PCR detection technology, fluorescence quantitative detection technology, TCT technology, HC2 detection technology, etc.) have the disadvantages of cumbersome operation methods, low sensitivity or specificity, etc.

Method used

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  • Nucleic acid composition for detecting HPV (Human Papilloma Virus) types as well as application and kit thereof
  • Nucleic acid composition for detecting HPV (Human Papilloma Virus) types as well as application and kit thereof
  • Nucleic acid composition for detecting HPV (Human Papilloma Virus) types as well as application and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The nucleic acid combination for detection of HPV typing provided in this example includes a first nucleic acid combination for detecting HPV 16 subtype and a second nucleic acid combination for detecting HPV 18 subtype. .

[0052] Wherein, the first nucleic acid combination includes a first primer pair and a first capture probe.

[0053] The first primer pair includes:

[0054] The upstream primer has a base sequence of: 5'-GAGCCCATTACAATATTGTA-3' (SEQ ID NO.1);

[0055] The downstream primer has a biotin label at its 5' end, and its base sequence is: Biotin-5'-GTCTTCCAAAGTACGAATGTCTACGTGTGTGCT-3' (SEQ ID NO.2).

[0056] The base sequence of the first capture probe is:

[0057] 5'-CGCTTCGGTTGTGCGTACAA-3' (SEQ ID NO.3)

[0058] The first capture probe is reverse complementary to the complementary strand target region (5'-TTGTACGCACAACCGAAGCG-3') of the first target nucleic acid fragment (SEQ ID NO.7) amplified by the first primer pair.

[0059] Additionally, the se...

Embodiment 2

[0069] This embodiment provides a kit, which includes the nucleic acid combination for HPV typing and detection provided in the above-mentioned embodiment 1.

[0070] The kit provided in this embodiment can be used to detect two subtypes of HPV 16 and HPV 18 in the sample to be tested. It has the characteristics of high sensitivity, good specificity and convenient operation.

Embodiment 3

[0072] This embodiment provides a kit, which includes the nucleic acid combination for HPV typing detection provided in Example 1 above, and also includes: PCR reaction buffer, dNTPs, Taq DNA polymerase and Mg 2+ solution.

[0073] The kit provided in this embodiment can be used to detect two subtypes of HPV 16 and HPV 18 in the sample to be tested. It has the characteristics of high sensitivity, good specificity and convenient operation.

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Abstract

The invention discloses a nucleic acid composition for detecting HPV (Human Papilloma Virus) types as well as application and a kit thereof and belongs to the technical field of gene detection. The nucleic acid composition for detecting HPV types comprises a first nucleic acid composition used for detecting HPV16 subtypes and a second nucleic acid composition used for detecting HPV18 subtypes, wherein the first nucleic acid composition comprises first primer pair expressed as SEQ ID NO.1-2 and a first capture probe expressed as SEQ ID NO. 3; the second nucleic acid composition comprises a second primer pair expressed as SEQ ID NO.4-5 and a second capture probe expressed as SEQ ID NO. 6. The nucleic acid composition takes PCR and EFIRM technologies as base, is capable of detecting classes of the HPV subtypes in the samples with high sensitivity and high specificity, has the characteristics of short detection time and low detection cost, and can be applied to a large amount of clinical detection and large-scale epidemiology screening.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a nucleic acid combination for HPV typing detection and its application and kit. Background technique [0002] Cervical cancer is one of the most common malignant tumors of the female reproductive tract. Studies have shown that persistent infection and multiple infections of high-risk human papillomavirus (HPV) are one of the important causes of cervical cancer. The results of the study showed that the presence of high-risk HPV DNA was detected in 99.7% of cervical cancer patients. [0003] There are more than 200 types of HPV that have been discovered, mainly including low-risk types and high-risk types. Among them, the two subtypes of HPV 16 and HPV 18 are the most common clinically. These two types of viruses have the highest probability of appearing in patients with cervical cancer. The correlation between different types and different diseases is also different. Low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/6816C12Q1/708C12Q2531/113C12Q2565/519C12Q2563/125
Inventor 廖玮莫亚勤吕佳韩林晓燕张晨光
Owner 北京易活生物科技有限公司
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