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Method for establishing muscle tissue damage repairing animal model

A damage repair and animal model technology, applied in medical science, surgery, diagnosis, etc., can solve the problems of affecting regeneration research, high cost, and inaccurate damage.

Inactive Publication Date: 2017-06-09
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, animal models commonly used in muscle tissue engineering include hereditary myopathy models, denervation models, and frozen models, but they have different degrees of shortcomings, such as unstable models, high costs, and inaccurate damage (hereditary myopathy) , Denervation affects regeneration research, long modeling period (denervation model) or difficult to control the extent of damage (freezing model)

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  • Method for establishing muscle tissue damage repairing animal model
  • Method for establishing muscle tissue damage repairing animal model
  • Method for establishing muscle tissue damage repairing animal model

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Embodiment Construction

[0019] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention.

[0020] The invention provides a method for establishing an animal model of muscle tissue damage repair, comprising the following steps:

[0021] Step 1, after anesthetizing the model animal experimental mouse, fix it in a prone position, and disinfect the hind limbs of the anesthetized experimental mouse;

[0022] Step 2, incision of the skin and subcutaneous tissue of the sterilized hindlimb in sequence, exposing the gastrocnemius muscle and separating the lower part of the gastrocnemius muscle;

[0023] Step 3: after one side of the gastrocnemius muscle is exposed, the electrodes are used to clamp the one side of the gastrocnemius muscle, and a pulsed electric field is ...

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Abstract

The invention belongs to the field of animal model construction, and specifically discloses a method for establishing an animal model for repairing muscle tissue damage, comprising the following steps: first anesthetizing the model animal, fixing it in a prone position, and disinfecting the hind limbs of the anesthetized experimental rat; Then, the skin and subcutaneous tissue of the sterilized hindlimb were incised sequentially through surgery to expose the gastrocnemius muscle and separate the lower part of the gastrocnemius muscle; finally, an electrode was used to clamp the gastrocnemius muscle on the exposed side, and a pulsed electric field was applied to the gastrocnemius muscle to establish an animal model of muscle tissue damage repair. . The present invention uses irreversible electroporation to establish an animal model of muscle tissue damage repair, which can provide a technical method that is simple, easy to implement, low in cost, short in modeling time, stable in model, accurate in damage, and accurate in scope and controllable, and can meet the requirements of destroying muscle cells at the same time. Requirement to preserve extracellular matrix structure.

Description

technical field [0001] The invention relates to the field of animal model construction, in particular to a method for establishing an animal model for muscle tissue damage repair. Background technique [0002] Stem cells in adult individual tissues are mostly in a dormant state under normal conditions. When a series of injuries such as trauma, inflammation, degenerative diseases and other consumptive diseases occur in the body, the tissue can maintain a certain degree of regeneration under pathological conditions or induced by external factors. ability. Muscle tissue has its own precursor cells, that is, muscle satellite cells. The muscle satellite cells attached to the surface of muscle cells have the nature of stem cells. When muscle cells are damaged, muscle satellite cells can proliferate and differentiate, and participate in the repair of muscle cell structure and function. However, the specific molecular mechanism is still unclear. As an important research tool for m...

Claims

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Application Information

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IPC IPC(8): A61B90/00
Inventor 李晓祥樊俊俊杨春宝李钊孙嗣国钱济先
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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