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Artificial fertilization method for loaches

A technology of artificial fertilization and loach, applied in fish farming, application, climate change adaptation, etc., can solve the problems of low fertilization rate and survival rate, and limit the development of loach breeding scale, and achieve high fertilization rate and high survival rate

Inactive Publication Date: 2017-06-23
重庆华楷农业科技有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the fertilization rate and survival rate of loach artificial insemination have always been low, which directly limits the scale development of loach farming.

Method used

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Experimental program
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Embodiment Construction

[0010] The present invention will be further described below in conjunction with embodiment: a kind of loach artificial insemination method may further comprise the steps:

[0011] 1) Seed selection: in March-October of each year, select the loach of 250-350g as kind of loach, wherein the proportioning of male loach and female loach is 1:6; Male loach and female loach are put into hatching bucket respectively;

[0012] 2) Oxygenation: 24 hours after the male loach and female loach were placed in the hatching bucket, they were injected with an oxytocin solution, which included 1 part of dioconone (DOM) for injection, 1 part of luteinizing hormone-releasing hormone A2 (LHRH-A2) for injection, 1 part of chorionic gonadotropin (HCG), 30 parts of glucose injection; each loach was injected with 0.4ml; after injection, the male and female loach were divided into standing tanks;

[0013] 3) Fertilization: 13-15 hours after the injection of oxytocin solution, squeeze the eggs for the f...

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Abstract

The invention discloses an artificial fertilization method for loaches. The artificial fertilization method for loaches comprises the following steps: 1) seed selection; 2) spawning induction: male loaches and female loaches are placed into a hatching barrel for 24 hours and injected with spawning induction liquid; 3) fertilization: eggs are extruded for female loaches after injection of spawning induction liquid for 13-15 hours and testes of male loaches are taken out; testes mucosae is added with a dextrose solution for 1 minute and residual seminal vesicle is removed; seminal fluids are absorbed into an injection pipe; female loaches are placed into an egg holding device and injected with seminal fluids while a disinfected feather is utilized for stirring 1 minute; oosperms are poured into aerated mineral water and stirred for 2 minutes. By adopting the artificial fertilization method for loaches, fertilization rate of loaches is high and survival rate of loaches is also high.

Description

technical field [0001] The invention relates to an artificial insemination method for loach. Background technique [0002] Loach (Misgurnus anguillicaudatus) is a common small freshwater fish in my country. Its meat is tender, delicious, rich in nutrition, and has high medicinal value. It is known as "ginseng in water". In recent years, the market demand for loach has been increasing day by day. However, due to years of overfishing and the deterioration of the ecological environment in natural waters, the resources of wild loach in my country are facing the danger of depletion. Since the 1990s, the breeding and aquaculture industry of loach has been gradually developed in my country, and it has become an important aquatic economic fish in our country. As a new small economic fish in our country, loach has broad development prospects. Therefore, the genetic breeding research of loach has important economic significance. However, in the prior art, the fertilization rate and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 黄昭伟
Owner 重庆华楷农业科技有限公司
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