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Inhalable siRNA against influenza virus infection and its application

A technology for influenza virus and influenza, applied in the direction of antiviral agents, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as inability to meet influenza virus, avoid incompatibility problems, reduce use costs, and have high reliability Effect

Active Publication Date: 2019-11-12
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to the two siRNAs originally designed by Ge et al., siRNAs targeting influenza virus proteins include PB1 (1632-1652), PA (ps-PA496), M2 (psM-950) and M (331-351), but With the continuous mutation of influenza virus and the emergence of highly pathogenic avian influenza, the known siRNA can no longer meet the needs of people to fight against influenza virus

Method used

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  • Inhalable siRNA against influenza virus infection and its application
  • Inhalable siRNA against influenza virus infection and its application
  • Inhalable siRNA against influenza virus infection and its application

Examples

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Embodiment 1

[0061] Embodiment 1, siRNA and its anti-influenza virus activity

[0062] 1 Materials and methods

[0063] 1.1 Materials

[0064] 1.1.1 siRNA

[0065]By comparing the gene sequences of influenza A virus strain A / WSN / 33, fragments that meet the design requirements of siRNA were extracted one by one from the beginning, and then BLAST was used to determine the homology of the fragments, and 10 siRNAs were selected for the next experiment. The sequence of one chain of each siRNA is shown in Table 1, and the sequence of the other chain is completely reverse complementary to the corresponding sequence listed in Table 1. Wherein, the nucleotide sequence from the 5' end to the 3' end of one chain of siPB2-1 is SEQ ID No.1 in the sequence listing, and the nucleotide sequence from the 3' end to the 5' end of the other chain of siPB2-1 The sequence is SEQ ID No.2 in the sequence listing; one chain sequence of siPB2-2 is SEQ ID No.3 in the sequence listing from the 5' end to the 3' end...

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Abstract

The present invention discloses anti-influenza virus infection siRNA capable of being subjected to atomizing inhalation, and applications thereof. According to the present invention, the siRNA is a double-stranded RNA molecule named siPB2-1, siPB2-2, siPB1-2 or siNS-1, the sequence of one chain of the siPB2-1 is represented by SEQ ID No.1 in the sequence table, the sequence of one chain of the siPB2-2 is represented by SEQ ID No3. in the sequence table, the sequence of one chain of the siPB1-2 is represented by SEQ ID No.5 in the sequence table, and the sequence of one chain of the siNS-1 is represented by SEQ ID No.7 in the sequence table; and the EC50 and the consumption in the in vivo mouse experiment of the siRNA are lower than the the EC50 and the consumption reported in the literature, such that the use cost can be substantially reduced by using the anti-influenza virus infection siRNA as the anti-influenza virus drug, and the inhibition effects can be provided for influenza viruses type A and influenza viruses type B.

Description

technical field [0001] The invention relates to an anti-influenza virus infection siRNA capable of nebulization and inhalation in the field of medical biotechnology and application thereof. Background technique [0002] Influenza virus is the most important pathogen that causes acute respiratory infectious diseases in humans. It is mainly transmitted through air droplets. It has the characteristics of fast transmission, high incidence and often serious complications. Influenza is prevalent every year in autumn and winter in temperate regions, causing quite high morbidity and mortality, and seriously threatening human health. It is estimated that around 250,000 to 500,000 people die from seasonal influenza each year worldwide. Since influenza virus can be transmitted directly or indirectly between different hosts, it is prone to genetic recombination to produce new subtypes, which may have disastrous consequences, such as several influenza outbreaks in human history, which k...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K9/72A61K31/713A61P31/16
CPCA61K9/0073A61K9/12A61K31/713C12N15/1131C12N2310/14
Inventor 张永欣赵斐王臻岑山
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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