Detection method of human myelin oligodendroglia glycoprotein self immune antibody
A technology for autoimmune antibodies and detection methods, applied in the field of detection of human myelin oligodendrocyte glycoprotein autoimmune antibodies, can solve the problems of inability to specifically bind antibodies, specificity and sensitivity, etc. problems, achieve important research and practical value, good specificity, and high detection efficiency
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[0019] 1 Construction of expression vector
[0020] Try a variety of MOG expression vectors, including pcDNA3.1-MOG, pcDNA3.1-MOG-GFP (express MOG and GFP fusion protein), pEGFP-IRES-MOG, etc., and compare the level of MOG antigen expressed on the cell surface by screening and comparing the expression vector pEGFP-IRES-MOG(expression vector2)( figure 1 ) The MOG antigen expressed on the cell surface is significantly higher than other expression vectors.
[0021] The construction method of pEGFP-IRES-MOG vector is: insert the internal ribosome entry site sequence (IRES) downstream of the green fluorescent protein (GFP) gene of the eukaryotic expression vector pEGFP vector (with CMV promoter), and clone after IRES The cDNA sequence encoding human full-length MOG was obtained. The human calnexin gene was cloned into the pcDNA3.1 vector to obtain the pcDNA-clanexin expression vector.
[0022] 2The chaperone protein calnexin promotes the membrane expression of MOG antige...
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