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Method for preventing saprolegniasis of fish fertilized eggs

A technology for fertilized eggs and saprolegnia, applied in the field of pure traditional Chinese medicine, can solve the problems of lack of high efficiency and effective dose, difficulty in obtaining experimental materials and sample quantity, etc.

Inactive Publication Date: 2017-10-24
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemicals such as humic acid, formalin, hydrogen peroxide, ozone, chlorine dioxide, bronopol, and sodium chloride used in production are used to prevent saprolegniasis, but they lack high efficiency and performance under laboratory conditions. The effective dose cannot effectively prevent saprolegniasis from conventional fertilized eggs and fertilized eggs operated by genetic engineering, which makes it difficult to obtain experimental materials and sample numbers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The present embodiment uses the doxycycline hydrochloride of 5ppm, and medaka and zebrafish eye development rate is 95%-100%, and hatching rate is 92-96%; Use the doxycycline hydrochloride of 10ppm, the medaka and zebrafish eye development rate The rate was 95-100%, and the hatching rate was 95%-98%. No matter whether the fertilized eggs of medaka and zebrafish were treated with 5ppm or 10ppm, no fertilized eggs infected by saprolegniasis were found. For the control group, the fertilized eggs infected by Saprolegnia accounted for 30%-40%, and some batches of fertilized eggs were as high as 45-50%; the average hatching rate was 45%-50%.

Embodiment 2

[0020] The present embodiment uses the doxycycline hydrochloride of 5ppm to process the fertilized eggs of the crocodile crocodile, and the rate of eye development is 55-85%, and the hatching rate is 40-50%; , the rate of eye formation is 55-85%, and the hatching rate is 55-65%; using 20ppm of doxycycline hydrochloride to treat the fertilized eggs of the crocodile, the rate of eye formation is 70-85%, and the hatching rate is 65-75%. For the control group of crocodile, the fertilized eggs infected by saprolegnia accounted for 60%-80%, and some batches of fertilized eggs were as high as 95-100%; the hatching rate was 5%-15%. The rate of eye formation and hatching rate of fertilized eggs of Doxycycline valschii treated with 20ppm doxycycline hydrochloride were significantly higher than the corresponding indicators of the treatment group and the control group using 5ppm.

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PUM

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Abstract

The invention provides a method for preventing saprolegniasis of fish fertilized eggs in order to overcome shortcomings in the prior art. The method includes: using a fine balance scale to accurately weigh 0.5g of doxycycline hydrochloride powder which is dissolved in 200ml of water to be figured into 0.25% of a stock solution; using a transfer pipette to accurately add 0.5ml (5ppm) or 1.0ml (10ppm) or 2.0ml (20ppm) of the stock solution of doxycycline hydrochloride to a culture vessel containing the fertilized eggs and 250ml of aerated tap water for hatching. The method is simple in operation, the saprolegniasis is effectively prevented, and hatching rate of the fertilized eggs is increased.

Description

technical field [0001] The invention belongs to pure traditional Chinese medicine, in particular to a method for preventing saprolegniasis of fish fertilized eggs. Background technique [0002] Fertilized eggs of cold-water fish and other fish, especially rare fish fertilized eggs and under special conditions, such as fertilized eggs microinjected with exogenous genes in genetic engineering, can be stored in places with relatively low water temperature (4-17°C). Under conditional cultivation and incubation, due to the limitation of experimental conditions and improper operation and management, no effective disinfection measures are taken, and it is easy to be infected by saprolegniasis. In factory production, when the fertilized eggs suffer from saprolegniasis, the propagation speed is very fast, and after 1-4 hours, it can cause large batches or even up to 100% mortality; Once the fertilized eggs of medaka, zebrafish and crocodile get saprolegniasis, they can cause 35%-95%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/13A01K61/17
CPCA01K61/13A01K61/17Y02A40/81
Inventor 苏宝锋尚梅梁利群
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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