Method for rapid identification of albumin and globulin products

A technology for albumin and globulin, which is applied in the field of rapid identification of albumin and globulin products. It can solve the problems of long background decolorization time and achieve the effects of reducing decolorization time, improving efficiency and saving time.

Inactive Publication Date: 2017-11-10
HUALAN BIOLOGICAL ENG CHONGQING +1
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a method for quickly identifying albumin and globulin products, which solves the problem of long decolorization time for the background after the precipitation line is produced in the existing method

Method used

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  • Method for rapid identification of albumin and globulin products
  • Method for rapid identification of albumin and globulin products
  • Method for rapid identification of albumin and globulin products

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A method for quickly identifying albumin and globulin products according to the present invention comprises the following steps:

[0025] S1: Drill a measurement hole at the top of the negative 1 / 3 of the pre-prepared agarose gel plate, and drill a control hole at the bottom, add 10 μl of product solution and 1 drop of bromophenol blue indicator solution to the measurement hole; add normal to the control hole 10 μl of human serum or human plasma and 1 drop of bromophenol blue indicator solution;

[0026] In order to make the outline of the precipitation line produced by the subsequent reaction clearer and easier to observe, the aperture diameters of the measurement wells and the control wells are both 3 mm, and the distance between the measurement wells and the control wells is 13 mm to 14 mm.

[0027] Wherein, the agarose gel plate is made by the following method: a, take 1.5g of agarose, add 50ml of water and 50ml of electrophoresis buffer, heat and swell to form a 1....

Embodiment 2

[0043]In Example 1, the test product has been determined to be human plasma as the raw material, and the protein type is distinguished. The double diffusion method in the "Chinese Pharmacopoeia" can distinguish human plasma from the plasma of other animals as a judgment of the specificity of protein products. This method can also be used to identify blood coagulation factor products, which is a necessary identification process that needs to be done before identifying protein types. It also uses the concentration ≥ 75% ethanol as mentioned in Example 1 to replace the "Chinese Pharmacopoeia" decolorizing solution.

[0044] The method for distinguishing human plasma (coagulation factor products) comprises the following steps:

[0045] 1. Use the agarose gel plate made in Example 1, then punch holes on the agarose gel plate, the hole diameter is 3mm, the hole distance is 3mm, and form a square matrix distribution;

[0046] The number of square formations can be expanded as needed...

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Abstract

The invention relates to a method for quickly identifying albumin and globulin products, which comprises the following steps: S1: punch measurement holes and control holes on an agarose gel plate, add 10 μl of product solution and bromophenol blue indicator to the measurement holes Add 1 drop of normal human serum or human plasma and 1 drop of bromophenol blue indicator solution to the control well; S2: Electrophoresis at a constant voltage of 100V for about 2 hours, and dig a 3-4mm wide depression between the measurement well and the control well Tank, add human serum antibody or human plasma antibody into the tank and spread it in a wet box; S3: fully soak the agarose gel plate with physiological sodium chloride solution, and stain it after soaking; Decolorize in the decolorization box with % ethanol, so that the background is decolorized and the precipitation line is clearly visible; S5: compare with the reference substance, and identify the protein type contained in the product solution. After using the method of the invention, the identification time for proteins is reduced from 4-5 days to 1-2 days, which greatly improves the efficiency of identification experiments.

Description

technical field [0001] The invention relates to the technical field of bioassay, in particular to a method for rapidly identifying albumin and globulin products. Background technique [0002] The 3403 immune double diffusion method and 3404 immunoelectrophoresis method in the fourth part of "Chinese Pharmacopoeia" (2015 edition) introduce the specificity and identification methods of protein products. After a certain period of time, the precipitation line of immune complexes is formed between the antigen and the antibody, which can check the specificity of the test product, and judge whether the test product is human plasma or serum according to its specificity. Then the test product is separated into two antigens of the zone by electrophoresis, and then two-way immunodiffusion is carried out with the corresponding antibody. Also at a certain concentration and ratio, a visible precipitation line (arc) will be formed, and the precipitation will be separated. The line is comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N33/6854
Inventor 王猛张宝献余姣姣刘刚秀杨柳
Owner HUALAN BIOLOGICAL ENG CHONGQING
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