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Culture method of astragalus mongholicus germchits

A cultivation method, technology of Astragalus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effect of less labor input, sufficient carbon dioxide and oxygen, and high quality

Active Publication Date: 2017-12-01
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports and related patents on cultivating Astragalus seedlings using intermittent submerged bioreactors. The present invention will disclose a new technology for the production of Astragalus seedlings

Method used

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  • Culture method of astragalus mongholicus germchits
  • Culture method of astragalus mongholicus germchits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present invention is a kind of cultivation method of astragalus seedling, and this method comprises the following steps:

[0034] (1) Explant preparation: In a sterile operating table, cut the solid-cultured Astragalus tissue cultured seedlings into stems containing 2-3 leaf buds, and inoculate 100 stems of Astragalus into sterilized and cooled astragalus containing 1L In the triangular flask of liquid medium, the formula of the medium is: MS+IBA0.05mg / L+6-BA0.5mg / L+sucrose 30g / L pH5.8~6.0, after the inoculation is completed, put the triangular flask into the culture room Cultivate for 3 days;

[0035] (2) Proliferation culture: In the aseptic operating table, pour the explants prepared in step (1) together with the liquid medium into the sterilized and cooled reactor tank, and connect the tank to the reaction tank. In the instrument system, set the immersion frequency to 5min / 4h, and cultivate in the culture room for 15 days;

[0036](3) Rooting culture: In the a...

Embodiment 2

[0039] The present invention is a kind of cultivation method of astragalus seedling, and this method comprises the following steps:

[0040] (1) Explant preparation: In a sterile operating table, cut the solid-cultured Astragalus tissue cultured plantlets into stems containing 2-3 leaf buds, and inoculate 150 stems of Astragalus into sterilized and cooled 1L In the triangular flask of liquid medium, the formula of the medium is: MS+IBA0.07mg / L+6-BA0.3mg / L+sucrose 30g / L pH5.8~6.0, after the inoculation is completed, put the triangular flask into the culture room Culture for 5 days;

[0041] (2) Proliferation culture: In the aseptic operating table, pour the explants prepared in step (1) together with the liquid medium into the sterilized and cooled reactor tank, and connect the tank to the reaction tank. In the instrument system, set the immersion frequency to 8min / 6h, and cultivate in the culture room for 20 days;

[0042] (3) Rooting culture: In the aseptic operating table,...

Embodiment 3

[0045] The present invention is a kind of cultivation method of astragalus seedling, and this method comprises the following steps:

[0046] (1) Explant preparation: In a sterile operating table, cut the solid-cultured Astragalus tissue cultured seedlings into stems containing 2 to 3 leaf buds, and inoculate 120 stems of Astragalus into sterilized and cooled 1L In the triangular flask of liquid medium, the formula of the medium is: MS+IBA0.06mg / L+6-BA0.6mg / L+sucrose 30g / L pH5.8~6.0, after the inoculation is completed, put the triangular flask into the culture room Culture for 4 days;

[0047] (2) Proliferation culture: In the aseptic operating table, pour the explants prepared in step (1) together with the liquid medium into the sterilized and cooled reactor tank, and connect the tank to the reaction tank. In the instrument system, set the immersion frequency to 6min / 5h, and cultivate in the culture room for 18 days;

[0048] (3) Rooting culture: In the aseptic operating tab...

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Abstract

The invention provides a culture method of astragalus mongholicus germchits. The culture method is used for culturing by utilizing an intermittent submerged bioreactor; the culture method comprises the following steps: (1) preparing explants; (2) carrying out multiplication culture; (3) carrying out rooting culture. According to the culture method provided by the invention, the astragalus mongholicus germchits are cultured by utilizing an the intermittent submerged culture method for the first time; the culture method has the advantages of short germchit culture period, high germchit quality, good rooting effect and low production cost and a new technology is provided for culture and production of the astragalus mongholicus germchits.

Description

technical field [0001] The invention belongs to the field of seedling cultivation of traditional Chinese medicinal materials, and in particular relates to a method for cultivating astragalus seedlings, in particular to a method for cultivating astragalus seedlings by using an intermittent submerged bioreactor. Background technique [0002] Astragalus [ Astragalus membraneceus Bge. Var. mongholicus (Bge.) Hsiao] is a perennial herbaceous plant of the leguminous Astragalus genus. The root can be used as medicine. With the increasing sales of Astragalus health medicines, the demand for Astragalus membranaceus is increasing. Due to long-term over-harvesting, wild resources are almost exhausted, and Astragalus membranaceus seeds are hardened, and the germination rate is low, and artificial seed propagation is also affected. Therefore, the research on tissue culture and plant regeneration of Astragalus membranaceus is of great significance in the production of seedlings. The re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张本厚胡燕花陈集双张保钱陈国晶郝圣响
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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