An assay for the ability to assemble chromatin structures at specific sites in DNA sequences

A DNA sequence and site-specific technology, applied in the field of molecular biology, can solve the problems of cumbersome experimental steps, high experimental operation requirements, and high price, and achieve the effect of high specificity, high accuracy, and simple implementation process
CN107541558BActive Publication Date: 2021-03-23INNER MONGOLIA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INNER MONGOLIA UNIV OF SCI & TECH
Publication Date
2021-03-23

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Abstract

The invention relates to the field of molecular biology, in particular to a capacity detection method for assembling a chromatin structure on a specific site of a DNA sequence, and aims to solve the problem that an existing technology cannot detect the nucleosome occupying rate at a fixed site to determine the chromatin structure assembling capacity. The method is mainly to assemble the DNA sequence containing a specific restriction enzyme site into the chromatin structure; enzyme digestion is carried out through restriction enzyme to remove various types of protein, then electrophoresis detection is carried out, and the site and the nucleosome occupying rate of the DNA sequence near the site are quantitatively calculated to judge a local chromatin structure near the site. Therefore, the efficiency of assembling the chromatin structure on the specific site on the DNA sequence and the nucleosome assembling capacity are analyzed. The detection method is simple and quick in realizing process, and high in specificity and accuracy.
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Description

technical field

[0001] The application relates to the field of molecular biology, in particular to a method for detecting the ability to assemble chromatin structure at a specific site in a DNA sequence. Background technique

[0002] The nucleosome is the basic structural unit of eukaryotic chromatin. Two copies of each of the four histones H2A, H2B, H3, and H4 form a histone octamer, on which a DNA sequence of about 147 bp is wound in a left-handed helical manner 1.7 circles, constituting nucleosome core particles. The core particles are connected by 20-80bp DNA, which is further compressed and packaged into a 30nm chromatin secondary structure under the action of histone H1.

[0003] As a basic structural unit of eukaryotic chromatin, nucleosome and chromatin have dual functions of structure and function. The position of nucleosomes on genomic DNA and the local structure of chromatin regulate many biological processes including DNA replication, transcription, repair, rec...

Claims

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