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Psoriasis skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof

A skin repairing agent and extract technology, applied in the field of psoriasis skin repairing agent, to achieve the effect of less fibroblasts, inhibiting growth and increasing blood vessel density

Active Publication Date: 2018-01-09
上海亚睿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no report on the skin repair agent prepared by mixing stem cell extracts and traditional Chinese medicine extracts according to the present invention.

Method used

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  • Psoriasis skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof
  • Psoriasis skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof
  • Psoriasis skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 skin restoration agent of the present invention

[0037] Step 1: Preparation of umbilical cord mesenchymal stem cell extract

[0038] (1) Take out the umbilical cord tissue of the newborn, remove blood vessels, epithelial membranes and blood clots, separate Wharton's block, and cut it into 1mm 3 After size, put it into a 10cm sterile petri dish; digest with DMEM medium containing 0.1% collagenase II; after filtration, use DMEM medium containing 10% fetal bovine serum FBS for subculture, and replace it with serum-free medium on the third day Subculture in DMEM medium, once every 2 days; take a bottle of cells from passage 3 for subsequent treatment;

[0039] (2) digest the cells with DMEM medium containing 0.05M EDTA, and wash with PBS solution;

[0040] (3) The cells were resuspended in serum-free DMEM medium, and lysed and broken by ultrasonic (120W, 15S*20 times, 15S interval), the broken product was centrifuged at 12000rpm for 15 min...

Embodiment 2

[0049] The preparation of embodiment 2 skin restoration agent of the present invention

[0050] 1. Experimental method

[0051] (1) Primary culture of umbilical cord mesenchymal stem cells;

[0052] (2) Umbilical cord mesenchymal stem cells were mixed with 2*10 5 cells / ml into a 12-well plate for experiments, and adherent culture for at least 24 hours;

[0053] (3) Add the skin repair agent prepared in Example 1 of the present invention (the mass concentration is respectively 0%, 0.5%, 1%, 2%, 4%, 10% and 15%), and add the cell damage agent bleomycin as comparison;

[0054] (4) Perform flow cytometry detection on the cells to detect cell apoptosis and cell viability (CCK8 method).

[0055] 2. Experimental results

[0056] For the CCK8 experiment of mesenchymal stem cells, the cytotoxicity of the skin repair agent of the present invention at a concentration of 4% is 6.0%, and the cytotoxicity at a concentration of 10% is 15.2%, indicating that its cytotoxicity is small, an...

Embodiment 3

[0057] Example 3 The cell experiment that the skin repair agent of the present invention inhibits the growth of fibroblasts and promotes the growth of epidermal stem cells and vascular endothelial cells

[0058] 1. Experimental method

[0059] (1) Primary culture of different cells, including fibroblasts, epidermal stem cells and vascular endothelial cells;

[0060] (2) Divide cells into 2*10 5 Spread into 12-well plates for experiments, 4 wells for each type of cells, and culture on the wall for at least 24 hours;

[0061] (3) Add the skin repair agent (mass concentration 4%) of the present invention to each kind of cell respectively, and add bleomycin (final concentration is 2 μ g / ml) as the cell damage agent, the concrete adding method is:

[0062]

[0063] (4) Perform flow cytometry detection on all cells to detect cell apoptosis and cell viability (CCK8 method).

[0064] 2. Experimental results

[0065] The results of cell apoptosis in each group are shown in Table...

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Abstract

The invention relates to a psoriasis skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract, and a method for carrying out traumatic repairing on psoriasisby utilizing the preparation. The method comprises the following steps: (1) culturing umbilical cord mesenchymal stem cells and preparing extract by utilizing technologies including ultrafiltration and the like; (2) mixing the sterile extract and special traditional Chinese medicine extract, and packaging; (3) applying the preparation to psoriasis pathogenic parts through methods including spraying, superficial injection and the like, and treating psoriasis through promoting tissue revitalization and killing fibroblasts. The method provided by the invention has the advantages of safety, high efficiency, small side effects, simplicity in production and low cost.

Description

technical field [0001] The invention relates to the technical field of medical medicine, in particular to a psoriasis skin repair agent prepared by mixing stem cell extracts and traditional Chinese medicine extracts. Background technique [0002] Psoriasis is a common, chronic skin disease prone to repeated attacks. In my country, the incidence rate of psoriasis is about 0.5%, and it is one of the most common skin diseases at present. Psoriasis is extremely harmful to patients, because psoriasis can cause repeated damage to the skin, is extremely difficult to cure, and is often accompanied by a large number of inflammatory symptoms, and even causes joint damage such as rheumatoid arthritis in severe cases. But at present, there is no perfect treatment method for the treatment of psoriasis, because the pathogenesis of psoriasis is relatively complicated, and it is difficult for a single treatment method to take care of all the treatment methods. [0003] Psoriasis is essent...

Claims

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Application Information

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IPC IPC(8): A61K36/888A61P17/06A61P17/02A61K35/28
Inventor 童强汪佳祺
Owner 上海亚睿生物科技有限公司
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