A method for isolation and identification of Streptococcus suis race

Abstract

The invention discloses a method for isolating and identifying races of Streptococcus suis, belonging to the technical field of races identification. Extract the total nucleic acid of microorganisms in the sample and construct a high-throughput sequencing library; find the variation sites on the genome; screen high polymorphic sites by sliding translation, and design multiple amplification primers on both sides of the candidate sites; use The single colony in the sample is isolated from the solid medium and further purified to obtain a monoclonal colony; the genomic DNA of the colony is extracted and then amplified and sequenced using the primers designed in the above steps; based on the sequencing results, the monoclonal colony is typed, with different Genotyped colonies were followed up as distinct races. This method does not need to know the number and abundance of races in the sample in advance, nor does it need the physiological and biochemical identification tests required in the traditional screening process. All small species, the process is simple, fast and standardized.

Description

technical field [0001] The invention belongs to the technical field of race identification, in particular to a method for separation and identification of a Streptococcus suis race. Background technique [0002] Streptococcus suis disease is an acute high fever infectious disease caused by different groups of Streptococcus. The epidemic is characterized by septicemia and meningitis in piglets, and arthritis in medium and large pigs. The condition of suis streptococcosis is complex, and the epidemic situation has no characteristics. Some preliminary diagnosis can be made according to clinical symptoms and pathological changes, and some require laboratory examination, and the pathogenicity of different races of pathogenic bacteria is different; Diagnosis should be distinguished from acute swine erysipelas, swine fever, and listeriosis. [0003] Races, also known as physiological races, strains, etc., refer to different groups of the same microorganism with no obvious differe...

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there are at least the following problems: since there is no obvious difference in morphology between the various races, it is still impossible to know how many races are contained in the sample and what race they are after obtaining the sample and doing isolation culture. Can only be identified by a series of isolation culture and physiological and biochemical tests

This leads to three problems, one is that some races will be missed; the other is that the picking needs to repeatedly perform physiological and biochemical analysis on multiple monoclonal colonies to screen the races; Disinfection of pig farms to kill all streptococcal races present

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