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Novel biological agent for treating drug resistant tuberculosis and preparation method thereof

A technology of biological agents and tuberculosis, applied in biochemical equipment and methods, microorganisms, pharmaceutical formulas, etc., can solve the problems of less progress, fatigue, and increased difficulty, and achieve the disappearance of cough and sputum symptoms, appetite and weight gain, and fatigue disappearing effect

Active Publication Date: 2018-03-20
苏州市第五人民医院
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Problems solved by technology

However, due to the long-term passage, the activity of BCG is reduced and the immune regulation is not effective after clinical use, which makes traditional immunotherapy and prevention gradually fall into a dilemma
The prevalence of multidrug-resistant tuberculosis has made the existing conventional chemotherapy methods weak
In recent years, with the increasing emphasis on tuberculosis, countries have invested a lot of money in the research of new anti-tuberculosis drugs and new vaccines, but little progress has been made and it is far from meeting clinical needs. Therefore, due to the characteristics of multidrug-resistant tuberculosis itself, The effect of combined application of chemically synthesized drugs is greatly reduced
[0004] In summary, the existing problems in the prior art are: low efficiency of chemically synthesized drugs for treating multidrug-resistant tuberculosis
The main reason is that multidrug-resistant Mycobacterium tuberculosis has developed resistance mutations to various existing anti-tuberculosis chemical drugs. Synthetic drugs make it harder to treat MDR-TB

Method used

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  • Novel biological agent for treating drug resistant tuberculosis and preparation method thereof
  • Novel biological agent for treating drug resistant tuberculosis and preparation method thereof
  • Novel biological agent for treating drug resistant tuberculosis and preparation method thereof

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preparation example Construction

[0026] Such as figure 1 As shown, the preparation method of the novel biological agent for the treatment of drug-resistant tuberculosis provided by the embodiments of the present invention comprises the following steps:

[0027] S101: Take peripheral blood from normal people, dilute it with PBS at a ratio of 1:2, and then perform Ficoll density gradient centrifugation. First add Ficoll, which has been placed at room temperature in advance, into the Fahrenheit tube, and then slowly add the diluted blood along the tube wall according to the ratio of Ficoll:diluted blood = 1:2 (the Ficoll tube is inclined at 45°, and the interface between Ficoll and the diluted blood should not be strongly shaking), and then centrifuged in a temperature-controlled centrifuge (1800rpm / min, 30min, 20-24°C). After centrifugation, the required PBMCs were obtained from the middle cloud layer. Use a pipette to carefully absorb the cloud layer cells, then wash and centrifuge with 10 times the volume o...

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Abstract

The invention belongs to the technical field of biological agents and discloses a novel biological agent for treating drug resistant tuberculosis and a preparation method thereof. The novel biologicalagent for treating drug resistant tuberculosis is a tuberculosis specific lymphocyte and is prepared by utilizing IL-1 alpha, IL-2, a CD3 monoclonal antibody and tuberculosis specific antigens (CFP10, ESAT6). Compared with the conventional tuberculosis treatment method, the novel biological agent disclosed by the invention is reported for the first time at home and abroad, and obvious curative effects are achieved when patients with pulmonary tuberculosis are treated by utilizing lymphocytes cultured in vitro. For patients subjected to cell therapy, the number of lymphocytes in peripheral blood is obviously increased, the sputum examination result is rapidly turned to be negative, pulmonary shadow is rapidly absorbed, the course of disease is shortened, the mental state is improved, and the appetite and weight are increased. The living quality of the patients in anti-tuberculosis therapy is obviously improved.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, in particular to a novel biological preparation for treating drug-resistant tuberculosis and a preparation method thereof. Background technique [0002] Tuberculosis caused by Mycobacterium tuberculosis infection is one of the major public health problems worldwide. WHO pointed out in its 2016 global tuberculosis report that in 2015, the number of new tuberculosis cases in the world increased by 10% compared with 2014, reaching 10.4 million cases, resulting in about 1.4 million deaths; the crisis of drug-resistant tuberculosis detection and treatment continued, 580,000 Only 125,000 (20%) of drug-resistant TB patients received treatment; three countries, India, China and Russia, accounted for 45% of all drug-resistant cases. Therefore, my country is one of the countries with a high incidence of tuberculosis, and it is also a country with a high incidence of drug-resistant tubercul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/26A61P31/06C12N5/0783
CPCA61K35/26C12N5/0636C12N2500/72C12N2501/2302C12N2501/24C12N2501/515C12N2501/998C12N2506/11
Inventor 胥萍徐俊驰吴妹英徐俊华宋华峰张建平叶志坚陈慧唐佩军赵静陈兴年时翠林吴敏娟朱晓燕王凤平
Owner 苏州市第五人民医院
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