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A Scale-up Metabolome Qualitative Method

A qualitative method, metabolomics technology, applied in the field of analytical chemistry, can solve problems such as lack of scale, and achieve the effect of large-scale qualitative

Active Publication Date: 2020-11-03
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

[0005] At present, there is no large-scale and systematic qualitative method for metabolome characterization. As a non-targeted fragmentation mode, in-source fragmentation has a good application prospect in the large-scale qualitative metabolome. The present invention relies on mass spectrometry in-source fragmentation technology, establish a large-scale metabolome qualitative method, and provide a new means for the metabolome characterization

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  • A Scale-up Metabolome Qualitative Method
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  • A Scale-up Metabolome Qualitative Method

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Embodiment 1

[0029] Taking plant leaves as an example, a large-scale qualitative study of its metabolites was carried out. The specific steps are: prepare a mixed plant sample, and perform ultra-high performance liquid chromatography-high resolution mass spectrometry analysis on the sample under different fragmentation voltages in the mass spectrometer source; obtain the peak table after peak matching of the original data, and obtain a characteristic from the peak table. For metabolites with neutral loss or characteristic product ions, search the mass spectrometry database to quickly lock the metabolite category and achieve large-scale characterization. For substances with commercially available standard samples, further standard sample verification can be carried out to confirm the qualitative results. Metabolites with specific neutral loss characteristics or characteristic product ions can use a single neutral loss, multiple neutral losses or neutral losses combined with multi-level mass...

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Abstract

The invention provides a large-scale metabolome qualitation method. By using a liquid chromatogram-high resolution mass spectrum non-targeting in-source dissociation technique, systematic large-scalequalitation is carried out on plant metabolome by using multiple strategies such as characteristic neutral loss, multiple neutral loss combinations, neutral loss with multi-stage mass spectrums and characteristic daughter ion recognition, laying a base for further study on variation of plant metabolome. Based on the advantages of high-resolution and high-sensitivity mass spectrums, large-scale characteristic structured metabolin is identified, the types of metabolin can be rapidly locked, the qualitative performance of a conventional mass spectrum method is widened, the metabolin qualitation efficiency is improved, the metabolome cover range is widened, particularly the difficulty of qualifying secondary metabolite of plants is partially solved, and meanwhile, reference can be provided forlarge-scale qualitation study of other living bodies.

Description

technical field [0001] The invention relates to the field of analytical chemistry, and is a new qualitative method for large-scale metabolomes based on ultra-high performance liquid chromatography-high resolution mass spectrometry. Background technique [0002] Metabolomics is a science that studies biological systems by examining the changes in their metabolites or their changes over time after a biological system (cell, tissue or organism) is stimulated or disturbed. In recent years, the sensitivity and resolution of liquid chromatography-mass spectrometry (LC-MS) technology have been developed rapidly, and the number of metabolites that can be detected by non-targeted metabolomics has been increasing. However, most of the metabolite structures still could not be identified. Metabolomics qualitative, especially large-scale qualitative methods, is one of the hot and difficult issues in current metabolomics research. The existing qualitative methods of metabolites include ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 许国旺张俊杰李在芳李丽丽路鑫赵春霞
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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