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Application of miRNA-4741 as diagnostic marker for primary liver cancer, and method for detecting miRNA-4741

A primary liver cancer, 1.mirna-4741 technology, applied in the field of medical diagnosis, can solve the problems of negative AFP, AFP diagnosis errors, and elevation

Inactive Publication Date: 2018-03-30
NANHUA UNIV
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Problems solved by technology

[0003] Alpha-fetoprotein (AFP) is currently the most widely used tumor marker for the diagnosis of liver cancer, but there are still diagnostic errors in AFP, for example, AFP in some patients with liver cancer is negative or slightly elevated

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  • Application of miRNA-4741 as diagnostic marker for primary liver cancer, and method for detecting miRNA-4741
  • Application of miRNA-4741 as diagnostic marker for primary liver cancer, and method for detecting miRNA-4741
  • Application of miRNA-4741 as diagnostic marker for primary liver cancer, and method for detecting miRNA-4741

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Embodiment Construction

[0029] The present invention will be further described below through specific embodiments.

[0030] Sera from 57 patients with confirmed primary liver cancer and 45 healthy individuals from physical examination were collected. 4ML of peripheral blood was collected from each group, and informed consent was obtained. Centrifuge at room temperature, 3600r, for 5 minutes, take the upper serum, and store it in a -80°C refrigerator for later use.

[0031] The RNA extraction method is as follows,

[0032] (1) Take 200ul of the upper serum sample, add 5 times the amount of QIAzol lysate, mix well, and let stand at room temperature (23-25°C) for 5 minutes;

[0033] ⑵Add 7ul miRneasy serum / plasma spike-in control;

[0034] (3) Add the same amount of chloroform as the sample, shake vigorously for 15 seconds, and let stand at room temperature (23-25°C) for 2-3 minutes;

[0035] (4) 12000Xg, 4°C, centrifuge for 15 minutes;

[0036] (5) Pipette 600ul of the upper layer, add 1.5 times the...

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Abstract

The invention relates to a method for detecting the expression level of circulating miRNA-4741, and an application of the miRNA-4741 in the diagnosis and prognosis of a liver cancer patient. The miRNA-4741 having a high expression level, used as the target marker of experiments, is found through carrying out high-throughput screening on the kind of the circulating miRNA in the blood plasma of a patient diagnosed with the liver cancer and comparing the miRNA with corresponding miRNA in the blood plasma of a healthy control. The expression level of the miRNA-4741 is higher than that of a controlgroup, and P is 0.001. An ROC curve analysis result shows that the usefulness AUC of the miRNA-4741 in the identification of normal people and a liver cancer patient is 0.765, the sensitivity is 75.4%, and the specificity is 66.7%. A survival curve analysis result shows that the life is short if the expression level is high, and the life is long if the expression level is low.

Description

technical field [0001] The invention belongs to the technical field of medical diagnosis, and relates to the application and detection method of MiRNA-4741 as a primary liver cancer diagnostic marker. Background technique [0002] Liver cancer is a common malignant tumor of the digestive system, and its mortality rate ranks third among all cancer patients, and its five-year survival rate is only 7%. high rate features. The etiology mainly includes: viral hepatitis B, chronic hepatitis and liver cirrhosis, etc. The occurrence of liver cancer is most closely related to the activation of proto-oncogenes and the inactivation of tumor suppressor genes. At present, the more effective methods are mainly surgery and liver transplantation, but usually there are no specific symptoms in the early stage of liver cancer, and once it is discovered, it will enter the late stage and lose the period of operation. At present, the 5-year survival rate of HCC patients with early surgical rese...

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 左建宏张楚红李金成
Owner NANHUA UNIV
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