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A kit, method and application for early diagnosis of tumor diseases

An early diagnosis and kit technology, applied in the field of diagnostic reagents for tumor diseases, can solve the problems of specific capture and detection of tumor cancer exosomes, time-consuming and labor-intensive problems

Inactive Publication Date: 2021-06-15
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the specific capture and detection of cancer exosomes is still difficult and time-consuming

Method used

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  • A kit, method and application for early diagnosis of tumor diseases

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Embodiment 1

[0073] Taking liver cancer as an example below, a preparation method of a kit for early diagnosis of liver cancer tumor diseases and a detection method using the kit are provided. The preparation method of the kit includes the following steps:

[0074] S1, Preparation of tumor exosome-specific capture system, including magnetic gold nanosphere complex solution with tumor exosome-specific ligand-modified Fe3O4 core and exosome-specific ligand-modified gold nanocage solution; in this embodiment 1, tumor exosomes refer to GPC3 high expression liver cancer exosomes GPC 3+ crExos;

[0075] S11, preparation of magnetic gold nanospheres

[0076] S111, add 12 mM (NH 4 ) 2 Fe(SO 4 ) 2 •6H 2 O solution 6 mL, stir the solution quickly, and then quickly add freshly prepared 4.17 mM H 2 o 2 solution, and make the solution H 2 o 2 with Fe 2+ The molar ratio is 1:8, the (NH 4 ) 2 Fe(SO 4 ) 2 •6H 2 O, regulates Fe 3 o 4 The particle size of the nanoparticles is 10 nm. Contr...

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Abstract

The invention belongs to the technical field of diagnostic reagents for tumor diseases, and specifically discloses a kit, method and application for early diagnosis of tumor diseases. The kit includes a tumor exosome-specific capture detection system and a molecular beacon fluorescence detection system; wherein, the tumor exosome-specific capture detection system includes tumor exosome-specific ligand-modified tetroxide The magnetic gold nanosphere complex solution of the triiron core and the gold nanocage solution modified by the exosome-specific ligand; the molecular beacon fluorescence detection system includes a molecular beacon probe with a fluorescent group, a fluorescent Fluorescence amplification substrate hybridization chain, buffer of the group. The kit and detection method of the present invention combine the specific capture analysis of tumor exosomes with the molecular beacon fluorescence detection method, which greatly improves the accuracy and reliability of early diagnosis of tumors.

Description

technical field [0001] The invention belongs to the technical field of diagnostic reagents for tumor diseases, and in particular relates to a kit for early diagnosis of tumor diseases, preparation and detection methods and applications. Background technique [0002] At present, the diagnosis of many diseases still faces severe challenges. Tumor is one of the main causes of death, and it is also a hot spot and difficulty in current research. In the early stage of cancer, the symptoms of patients are not obvious, and it is difficult to detect and detect. When cancer is diagnosed, the disease often has progressed to the middle and late stage, the prognosis is poor, and the survival period is short. Therefore, early detection and timely treatment are the key to effective prevention and treatment of tumor diseases. [0003] Exosomes are nanoscale vesicles with a diameter of approximately 50-150 nm that exist in blood and other body fluids. In 2015, "Nature" reported that exos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/682
CPCC12Q1/682C12Q2563/137C12Q2563/107C12Q2525/301C12Q2525/207
Inventor 胡玉荣吉梦飞李慧丽袁金秀段少峰徐鑫黄胜楠
Owner ZHENGZHOU UNIV
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