A kind of preservation method of liquid enzyme composition

A preservation method and composition technology, applied in the direction of biochemical equipment and methods, enzymes, enzyme stability, etc., can solve the problems of increased storage and transportation costs, inability to use at the same time, increased use costs, etc., achieve no toxic side effects, inhibit yellowing The effect of aspergillus

Active Publication Date: 2020-09-01
宁夏乙征生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, the property of acid protease to degrade protein also prevents it from being used simultaneously with other acid enzymes, otherwise the application effect of other acid enzymes and acid protease itself will be greatly reduced
In order to ensure the application effect during use, the current method is to store and use acid protease separately from other acid enzymes, which causes an increase in storage and transportation costs, and the separate use of the two also causes an increase in use costs

Method used

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  • A kind of preservation method of liquid enzyme composition
  • A kind of preservation method of liquid enzyme composition
  • A kind of preservation method of liquid enzyme composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preservation method of a liquid enzyme preparation composition of the present invention comprises the following steps:

[0026] (1) by weight percentage 30% acid cellulase, 20% acid mannanase, 20% acid xylanase, 0.5% chitosan and 0.5% xanthan gum at 50r / min Stir at a rotating speed for 5 minutes, then add 20% maltose and 9% glucose and continue stirring at a rotating speed of 50r / min for 20 minutes to obtain an enzyme preparation composition mixture I;

[0027] (2) Stir 50% acid protease and 40% glycerol by weight at 50r / min for 10min, then add 5% amino acid and 5% calcium propionate and continue stirring for 5min at 60r / min to obtain Enzyme preparation composition mixture II;

[0028] (3) Mix the enzyme preparation composition I obtained in step (1) and the enzyme preparation composition II obtained in step (2) at a weight ratio of 2:3, and immediately stir at 50r / min for 20min to obtain the final product liquid Enzyme composition.

Embodiment 2

[0030] A preservation method of a liquid enzyme preparation composition of the present invention comprises the following steps:

[0031] (1) by weight percentage 10% acid cellulase, 20% acid mannanase, 50% acid xylanase, 0.1% chitosan and 0.1% xanthan gum at 60r / min Stir at a rotating speed for 10 min, then add 5% maltose and 14.8% glucose and continue stirring at a rotating speed of 50 r / min for 20 min to obtain the enzyme preparation composition mixture I;

[0032] (2) 80% acid protease by weight percentage, 10% glycerin were stirred at 60r / min rotating speed for 10min, then added 5% amino acid and 5% calcium propionate and continued to stir at 60r / min rotating speed for 20min to obtain Enzyme preparation composition mixture II;

[0033] (3) Mix the enzyme preparation composition I obtained in step (1) and the enzyme preparation composition II obtained in step (2) at a weight ratio of 2:3, and immediately stir at 60r / min for 20min to obtain the final product liquid Enzyme ...

Embodiment 3

[0035] A preservation method of a liquid enzyme preparation composition of the present invention comprises the following steps:

[0036] (1) by weight percentage 20% acid cellulase, 10% acid mannanase, 40% acid xylanase, 0.5% chitosan and 0.5% xanthan gum at 55r / min Stir at a rotating speed for 10 minutes, then add 20% maltose and 9% glucose and continue stirring at a rotating speed of 50 r / min for 5 minutes to obtain an enzyme preparation composition mixture I;

[0037] (2) 80% acid protease by weight percentage, 10% glycerol were stirred at 50r / min rotating speed for 5min, then added 5% amino acid and 5% calcium propionate and continued to stir at 50r / min rotating speed for 20min, Obtain enzyme preparation composition mixture II; (3) mix the enzyme preparation composition I obtained in step (1) and the enzyme preparation composition II obtained in step (2) in a weight ratio of 2:3, and immediately mix them at 50r / min Stir for 20 minutes at a rotating speed to obtain the fin...

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Abstract

The invention belongs to the technical field of enzyme preparation application, and particularly provides a method for preserving a liquid enzyme preparation composition. The method comprises the following steps: preparing an enzyme preparation composition mixed solution I from 10-30wt% of acid cellulase, 5-20wt% of acid mannase and 20-50wt% of acid xylanase; preparing an enzyme preparation composition mixed solution II from 50-80wt% of acid protease and 10-40wt% of glycerol; uniformly mixing the enzyme preparation composition mixed solution I and the enzyme preparation composition mixed solution II according to a weight ratio of 2:3, and immediately stirring the mixture for 10-20min at the rotating speed of 50-60r / min to obtain a final product liquid enzyme preparation composition. The method provided by the invention can ensure that the activity of each of non-starch polysaccharase and a liquid complex enzyme of acid protease is not influenced when the non-starch polysaccharase and liquid complex enzyme are stored for a long time or are used simultaneously, thereby reducing the cost.

Description

technical field [0001] The invention belongs to the field of enzyme preparation applications, and in particular relates to a preservation method for a liquid enzyme preparation composition. Background technique [0002] Acid protease is an enzyme that catalyzes the hydrolysis of peptide bonds in proteins, and acid cellulase, acid xylanase, acid pectinase and acid protease are all proteins in nature, so other enzymes and acid protease are compounded together At the same time, the acid protease itself will undergo autolysis, and the acid protease will also degrade other acid enzymes, resulting in a rapid decline in the enzyme activities of other acid enzymes and acid proteases. The compound enzyme of acid cellulase, acid mannanase, acid xylanase and acid protease can be stored at 25°C for 7 days to produce acid cellulase, acid mannanase, acid xylanase and acid protease. The average enzyme activity loss of acid protease was more than 90%. [0003] In addition, the property of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96
CPCC12N9/2437C12N9/248C12N9/2488C12N9/50C12N9/96
Inventor 王双平苏二正陈龙
Owner 宁夏乙征生物工程有限公司
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