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KASP molecular marker of wheat scab resistance major QTL and applications of KASP molecular marker

A wheat scab and molecular marker technology, applied in the field of molecular biology, can solve the problems of large genetic distance between marker and QTL, small genetic effect, instability, etc., and achieve the effect of appropriate genetic distance and obvious stable genetic effect

Inactive Publication Date: 2018-05-04
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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Problems solved by technology

However, due to the small genetic effect (PVE<10%) and instability of most of the mapped QTLs, the large genetic distance between markers and QTLs, and the lack of simple high-throughput markers, most of the mapped QTLs cannot meet the requirements of molecular breeding. needs

Method used

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  • KASP molecular marker of wheat scab resistance major QTL and applications of KASP molecular marker
  • KASP molecular marker of wheat scab resistance major QTL and applications of KASP molecular marker
  • KASP molecular marker of wheat scab resistance major QTL and applications of KASP molecular marker

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Experimental program
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Embodiment

[0023] (1) Scab-resistant parent: Ji 5265 is a winter wheat variety bred by Hebei Academy of Agriculture and Forestry Sciences, and its resistance to scab can reach a high level.

[0024] (2) QTL mapping population: F1 was generated by crossing Ji5265 with wheat variety wheaton, which is highly susceptible to scab, and F2 was generated by self-crossing, and the F6 genetic population of Recombinant Inbreed Lines (RIL) was constructed by single-seed transmission.

[0025] (3) Identification of scab resistance: Under greenhouse conditions, single-flower instillation method was used to identify the resistance of parents and their derived RIL populations to scab. The scab inoculation pathogen used was the mixed race of scab provided by Jiangsu Academy of Agricultural Sciences. After the wheat flowered, the flowering spikelets in the middle were selected, each spikelet was inoculated with 10 μl of bacterial solution (about 1000 spores), and bagged to keep it moist for 48 hours. 14-...

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Abstract

The invention discloses a KASP molecular marker of a wheat scab resistance major QTL and applications of the KASP molecular marker. The major QTL is located on a long arm of a second chromosome of wheat, and is named as Qfhb-2DL. Qfhb-2DL comprises two linked KASP molecular markers, namely, GBS12056 and GBS10238, the positioning interval of Qfhb-2DL is within the 2.0cM interval of GBS12056 to GBS10238, 30.3% of phenotypic variation can be released, and the additive effect is -14.1. For the KASP molecular marker of the wheat scab resistance major QTL and the applications of the KASP molecular marker, the KASP molecular marker is obtained by excavating the tightly linked markers of the wheat scab resistance major QTL, the genetic effect of the QTL located by the KASP molecular marker is great and stable, the KASP molecular marker is in tight linkage with the target QTL, and the demand of molecular breeding can be met.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a KASP molecular marker of a major wheat scab resistance QTL and an application thereof. Background technique [0002] Wheat is an important food crop in my country, and its production is closely related to national food security. Wheat head blight is an important disease caused by Fusarium graminearum, which occurs worldwide. Different from other wheat diseases, the occurrence and prevalence of head blight not only seriously affect the yield and quality of wheat, but also the wheat grains infected by its pathogen can produce a variety of mycotoxins, such as deoxynivalenol (DON, Deoxynivalenol) and corn Zearalenone, etc., can cause human and livestock poisoning, making the susceptible wheat grains inedible and seriously affecting the production of wheat. Therefore, the European Union (European Union) and the FDA (Food and Drug Administration) of the United States have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6895
Inventor 宿振起李辉陈希勇王丽梅吕亮杰马乐
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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