KASP Molecular Marker and Its Application of the Major Qtl of Wheat Scab Resistance
A technology for wheat head blight and molecular markers, applied in the field of molecular biology, can solve the problems of large genetic distance between markers and QTLs, small genetic effects, and instability, and achieve the effect of appropriate genetic distance and obvious stable genetic effects
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[0023] (1) Scab-resistant parent: Ji 5265 is a winter wheat variety cultivated by the Hebei Academy of Agriculture and Forestry Sciences, and its resistance to scab can reach a high level.
[0024] (2) QTL mapping population: F1 was produced by crossing Ji5265 with wheat variety wheaton, which is highly susceptible to scab, and F2 was produced by self-crossing, and the F6 genetic population of Recombinant Inbreed Lines (RIL) was constructed by single-seed transmission.
[0025] (3) Identification of scab resistance: Under greenhouse conditions, single-flower instillation was used to identify the resistance of parents and their derived RIL populations to scab. The scab inoculation pathogen used was the mixed race of scab provided by Jiangsu Academy of Agricultural Sciences. After the wheat flowered, the flowering spikelets in the middle were selected, and each spikelet was inoculated with 10 μl of bacterial solution (about 1000 spores), and bagged to keep it moist for 48 hours....
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