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Method for quality breeding with maize o2, o16 and wx three-gene pyramided material

An o2o2o16o16wxwx, gene polymerization technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of poor nutritional quality, low content of lysine and amylopectin in common corn, and achieve stable quality traits, Choose from a wide range of effects that improve productivity

Inactive Publication Date: 2018-05-11
贵州省旱粮研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of lysine and amylopectin in common corn is low, and the nutritional quality is poor. Therefore, it is urgent to establish a method to quickly improve the nutritional quality of common corn, and provide effective means for corn quality improvement and breeding.

Method used

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  • Method for quality breeding with maize o2, o16 and wx three-gene pyramided material
  • Method for quality breeding with maize o2, o16 and wx three-gene pyramided material
  • Method for quality breeding with maize o2, o16 and wx three-gene pyramided material

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Test materials: maize o2, o16 and wx gene aggregation line QCL8002-11 (genotype is o2o2o16o16wxwx), common maize inbred line CML537 (genotype is O2O2O16O16WXWX).

[0033] 2. In the winter of 2012, the parents, the common corn inbred line CML537 and the gene aggregation line QCL8002-11 (o2o2o16o16wxwx), were planted in Ledong, Hainan, with 1 row each, and crossed at the flowering stage to obtain F1 generation seeds;

[0034] 3. In the spring of 2013, plant F1 generation seeds in Guiyang, one row, and use the common corn inbred line CML537 to backcross at the flowering stage to obtain BC1F1 generation seeds;

[0035] 4. In the winter of 2013, BC1F1 was planted in Ledong, Hainan, with 29 rows and 3-meter boxes, and a total of 306 individual plants were sampled at the seedling stage. Genomic DNA was extracted, and the individual plants with the genotype O2o2O16o16WXwx were selected using molecular markers phi061, phi112 and umc1141 , as a result, a total of 33 individual...

Embodiment 2

[0042] 1. Test materials: maize o2, o16 and wx gene aggregation line QCL8002-11 (genotype is o2o2o16o16wxwx), common maize inbred line CML530 (genotype is O2O2O16O16WXWX).

[0043] 2. In the winter of 2010, the parents were planted in Ledong, Hainan, the common corn inbred line CML530 and the gene aggregation line QCL8002-11 (o2o2o16o16wxwx), each with 1 row, and crossed at the flowering stage to obtain F1 generation seeds;

[0044] 3. In the spring of 2011, plant F1 generation seeds in Guiyang, one row, and use the common corn inbred line CML530 to backcross at the flowering stage to obtain BC1F1 generation seeds;

[0045] 4. In the winter of 2011, BC1F1 was planted in Ledong, Hainan, with 50 rows and 3-meter boxes. A total of 1010 individual plants were sampled at the seedling stage to extract genomic DNA, and the individual plants with the genotype O2o2O16o16WXwx were selected using molecular markers phi061, phi112 and umc1141 , as a result, a total of 32 individual plants ...

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Abstract

The invention discloses a method for quality breeding with a maize o2, o16 and wx three-gene pyramided material. The technical scheme is as follows: a common maize selfing line is hybridized with themaize o2, o16 and wx three-gene pyramided material, the common maize selfing line is used as a recurrent parent to be backcrossed twice, wherein BC1 / BC2 generations are subjected to molecular marker assisted screening in the seedling period, o2, o16 and wx three-gene pyramided single plants are screened out, lysine and amylopectin content determination is performed, and a high-quality waxy selfingline with high lysine is obtained. The method has great significance in maize quality gene pyramiding breeding practice and theoretical research and provides a reference basis for maize quality character genetic improvement.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding, and specifically relates to a method for quality breeding of maize o2, o16 and wx three-gene aggregate material. Background technique [0002] Corn is the main feed and food crop in the world. Quality is the main breeding goal, which directly affects the healthy development and eating taste of humans, livestock and poultry. The nutritional quality of corn kernels generally refers to the nutrients contained in corn kernels, such as the content and quality of protein, fat, starch and various vitamins and mineral elements. Among them, lysine in protein is an essential amino acid for monogastric animals such as humans, livestock and poultry, and the content of amylopectin in starch is closely related to the food quality. Common corn lysine content is low (below 0.3%), which cannot meet the nutritional quality requirements for human consumption and food processing (above 0.5%), and cannot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 王伟杨文鹏王明春张文龙
Owner 贵州省旱粮研究所
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