Method for extracting total flavonoid in pteridium aquilinum by ultrasound-assisted compound enzyme method

A compound enzymatic method and ultrasound-assisted technology, which can be used in drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of high energy consumption and long time, achieve simple process, convenient operation, and reduce blood vessel loss. Effect

Inactive Publication Date: 2018-06-01
WEST ANHUI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this extraction method takes a long time and consumes a lot of energy, and the ex

Method used

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  • Method for extracting total flavonoid in pteridium aquilinum by ultrasound-assisted compound enzyme method
  • Method for extracting total flavonoid in pteridium aquilinum by ultrasound-assisted compound enzyme method
  • Method for extracting total flavonoid in pteridium aquilinum by ultrasound-assisted compound enzyme method

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Embodiment 1

[0026] A method for extracting total flavonoids in bracken by an ultrasonic-assisted compound enzyme method of the present embodiment comprises the following steps:

[0027] (1) Take fresh young bracken leaves and petioles, kill them, dry them at 100°C until they are 80% dry, break them into 1cm pieces, put them into a high-speed pulverizer for crushing, and pass through a 35-mesh sieve to get bracken dry powder, continue for 100 ℃ drying to 99% dry;

[0028] (2) Put 50g of bracken dry powder into a 500mL Erlenmeyer flask, then add ethanol with a concentration of 70% at a ratio of 1:15, extract for 15min under the condition of ultrasonic power of 280W, and extract twice with an interval of 14min;

[0029] (3) Cool to room temperature, add 1.3% compound enzyme (cellulase: pectinase = 1:1), then extract at 50°C for 110 minutes, filter, collect the filtrate, and obtain the bracken flavonoid extract ;

[0030] (4) Put the above-mentioned bracken flavonoid extract into a rotary e...

Embodiment 2

[0034] A method for extracting total flavonoids in bracken by an ultrasonic-assisted compound enzyme method of the present embodiment comprises the following steps:

[0035] (1) Take fresh young bracken leaves and petioles, kill them, dry them at 100°C until they are 80% dry, break them into small pieces of 1 cm, put them into a high-speed pulverizer for crushing, pass through a 45-mesh sieve to get bracken dry powder, and continue for 100 ℃ drying to 99% dry;

[0036] (2) Put the bracken dry powder into a 500mL Erlenmeyer flask, then add ethanol with a concentration of 75% at a ratio of 1:20, extract for 25 minutes under the condition of ultrasonic power 320W, and extract 4 times with an interval of 16 minutes;

[0037] (3) Cool to room temperature, add 1.8% compound enzyme (cellulase: pectinase = 3:1), then extract at 55°C for 120min, filter, collect the filtrate to obtain the bracken flavonoid extract ;

[0038] (4) Take 50 g of the above-mentioned bracken flavonoid extra...

Embodiment 3

[0042] A method for extracting total flavonoids in bracken by an ultrasonic-assisted compound enzyme method of the present embodiment comprises the following steps:

[0043] (1) Take fresh young bracken leaves and petioles, kill them, dry them at 100°C until they are 80% dry, break them into 2cm pieces, put them into a high-speed pulverizer for crushing, and pass through a 40-mesh sieve to get bracken dry powder, continue for 100 ℃ drying to 99% dry;

[0044] (2) Put 50g of bracken dry powder into a 500mL Erlenmeyer flask, then add ethanol with a concentration of 73% at a ratio of 1:18, extract for 20min under the condition of ultrasonic power of 300W, and extract 3 times with an interval of 15min;

[0045] (3) Cool to room temperature, add 1.5% compound enzyme (cellulase: pectinase = 2:1), then extract at 53°C for 115min, filter, collect the filtrate to obtain the bracken flavonoid extract ;

[0046] (4) Put the above-mentioned bracken flavonoid extract into a rotary evapor...

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Abstract

The invention relates to the field of extraction of effective components of traditional Chinese medicines and discloses a method for extracting total flavonoid in pteridium aquilinum by an ultrasound-assisted compound enzyme method. The method comprises the following steps: (1) carrying out fixation on fresh pteridium aquilinum, drying, crushing and sieving to obtain dry powder; (2) putting the pteridium aquilinum dried powder into a conical flask; adding ethanol according to the ratio and extracting for 15 to 25min under the condition that the ultrasonic power is 280 to 320W; extracting for 2to 4 times, wherein the time interval is 14 to 16min; (3) cooling to room temperature and adding a compound enzyme; then extracting for 110 to 120min under the condition that the temperature is 50 to55 DEG C; filtering and collecting filtrate; (4) decompressing and concentrating until paste is formed, and freezing and drying; (5) carrying out polyamide column purification, freezing and drying. The method disclosed by the invention has the advantages that the ultrasound-assisted compound enzyme method is adopted; in the extracted pteridium aquilinum, the content of flavonoid is 9.0 to 9.2 percent, the content of rutin is 0.95 to 1.2 percent and the content of astragalin is 0.80 to 0.90 percent.

Description

technical field [0001] The invention relates to the field of extraction of effective components of traditional Chinese medicines, in particular to a method for extracting total flavonoids in bracken by ultrasonic-assisted compound enzyme method. Background technique [0002] Uric acid is the end product of human purine metabolism, which is produced by hypoxanthine and xanthine under the action of xanthine oxidase (XO). Due to the lack of urate oxidase in the human body, it cannot be further decomposed into soluble allantoin, which makes human beings very prone to hyperuricemia. Increased uric acid production and / or decreased excretion for any reason can lead to hyperuricemia. Usually hyperuricemia is considered a sign of gout, and according to statistics, about 5%-12% of hyperuricemia can eventually develop into gout. Studies in recent years have shown that hyperuricemia is not only the most important biochemical basis of gout, but also closely related to the occurrence of...

Claims

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Application Information

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IPC IPC(8): A61K36/12A61P19/06A61P9/00A61P13/12
CPCA61K36/12A61K2236/19A61K2236/333
Inventor 陈乃富谷仿丽姜雪萍陈乃东韩邦兴张自红
Owner WEST ANHUI UNIV
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