Method for determining content of isoferulic acid in largetrifoliolious bugbane rhizome

A technology of isoferulic acid and content, applied in the field of chemical detection, can solve the problems of long time and low efficiency, and achieve the effect of reducing viscosity, increasing diffusion and high precision

Inactive Publication Date: 2018-06-12
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, this method takes a long time, resulting in low efficiency

Method used

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  • Method for determining content of isoferulic acid in largetrifoliolious bugbane rhizome
  • Method for determining content of isoferulic acid in largetrifoliolious bugbane rhizome
  • Method for determining content of isoferulic acid in largetrifoliolious bugbane rhizome

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Experimental program
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Embodiment 1

[0030] 1. Instruments and reagents

[0031] 1.1 Instruments:

[0032] Electronic analytical balance (XA205DU), ASE350 fast solvent extractor (DIONEX, USA), ThermoU3000UHPLC liquid chromatograph

[0033] 1.2 Reagents:

[0034] Water: Class I water in accordance with GB / T 6682;

[0035] Propylene glycol: analytically pure;

[0036] Acetonitrile: chromatographically pure;

[0037] Quartz sand: the particle size is about 2mm.

[0038] 2. Method

[0039] 2.1 Preparation of reference substance solution: Take an appropriate amount of isoferulic acid reference substance, weigh it accurately, add methanol to make a solution containing 40ug per 1ml, and obtain it.

[0040] 2.2 Preparation of the test solution

[0041] 2.2.1 Pharmacopoeia method of 2015 edition

[0042] Take about 0.5g of the powder of this product (through the No. 2 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 25ml of 10% propylene glycol, seal it tightly, weigh it, heat to...

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Abstract

The invention discloses a method for determining the content of isoferulic acid in largetrifoliolious bugbane rhizome, which belongs to the field of chemical assay. The invention is aimed at providinga method for extracting isoferulic acid in largetrifoliolious bugbane rhizome by ASE (Accelerated Solvent Extraction) method which can shorten consumed time by multiple times and does not affect a final extraction result, the method is combined with HPLC (High Performance Liquid Chromatography), optimal parameters are set, and thereby the content of isoferulic acid in largetrifoliolious bugbane rhizome can be accurately determined. The method adopts ASE to extract ground largetrifoliolious bugbane rhizome by using propylene glycol, and collects propylene glycol extract; and HPLC is adopted todetermine the content of isoferulic acid in the propylene glycol extract. The method can substitute for the method recorded in the pharmacopoeia.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to a method for measuring the content of isoferulic acid in Cimicifuga. Background technique [0002] The 2015 edition of the Pharmacopoeia records that the preparation method of the test substance required for the determination of the content of isoferulic acid in Cimicifuga is as follows: take about 0.5g of the powder of this product (passed through a No. 2 sieve), accurately weigh it, and place a stopper In the Erlenmeyer flask, add 25ml of 10% propylene glycol precisely, seal it tightly, weigh it, heat it to reflux for 2.5 hours, let it cool, weigh it again, make up the lost weight with 10% propylene glycol, shake it well, filter it, and take the continuation Filtrate, that is. Centrifuge at 15000r / min for 3min, take the supernatant, and enter the LC assay. [0003] However, this method takes a long time, so the efficiency is low. Contents of the invention [0004] In view...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 张云平王华
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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