Method for determining spinosin content in spina date seed by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography)

A technology for spinosin and spinosin in kernels, which is applied in the field of ASE-HPLC determination of spinosin content in sour jujube kernels, can solve the problems of high professional level requirements for technicians, long reflux time, etc. Good repeatability, lower surface tension, increased diffusion effect

Inactive Publication Date: 2018-06-15
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0003] This method takes a long time to reflux and requires a high professional level of technicians

Method used

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  • Method for determining spinosin content in spina date seed by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography)
  • Method for determining spinosin content in spina date seed by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography)
  • Method for determining spinosin content in spina date seed by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography)

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Experimental program
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Embodiment 1

[0030] 1. Instruments and reagents

[0031] 1.1 Instruments: electronic analytical balance (XA205DU), ASE350 fast solvent extractor (DIONEX, USA), Thermo U3000UHPLC liquid chromatograph.

[0032] 1.2 Reagents:

[0033] Water: Class I water in accordance with GB / T 6682;

[0034] Acetonitrile: chromatographically pure;

[0035] n-Hexane, ethanol: analytically pure.

[0036] 2. Method

[0037] 2.1 Preparation of reference solution:

[0038] Take an appropriate amount of spinosin reference substance, weigh it accurately, add ethanol to make a solution containing 0.2 mg per 1 ml, and obtain it.

[0039] 2.2 Preparation of the test solution

[0040] 2.2.1 "Chinese Pharmacopoeia" 2015 Edition Part I Preparation method for the test product:

[0041] Take about 1g of this product powder (passed through a No. 4 sieve), weigh it accurately, put it in a Soxhlet extractor, add an appropriate amount of petroleum ether (60-90°C), heat and reflux for 4 hours, discard the petroleum ethe...

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Abstract

The invention discloses a method for determining spinosin content in spina date seed by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography), belongs to the field of chemical detection, and aims to provide a method that combines an ASE extraction method with a HPLC detection method, is short in time consuming and can achieve a desired effect. The method adopts the ASEmethod to extract spina date seed powder, wherein the ASE method comprises: first extracting with n-hexane, then extracting with ethanol, and collecting an ethanol extract; and the HPLC method is usedto measure the spineolin content in the ethanol extract. The method can replace a pharmacopoeia method to determine the spinosin content in the spina date seed, and monitor the the spinosin content in the spina date seed.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to an ASE-HPLC method for measuring the content of spinosin in jujube kernels. Background technique [0002] "Chinese Pharmacopoeia" 2015 Edition Part One The method for preparing the test product is to take about 1g of the powder of this product (passed through a No. 4 sieve), accurately weigh it, put it in a Soxhlet extractor, add an appropriate amount of petroleum ether (60-90°C), and heat Reflux for 4 hours, discard the petroleum ether liquid, evaporate the solvent from the dregs, transfer to a conical flask, add 20ml of 70% ethanol, heat and reflux for 2 hours, filter, wash the filter residue with 5ml of 70% ethanol, combine the washing liquid and the filtrate , recover the solvent to dryness, add ethanol to the residue to dissolve, transfer to a 5ml measuring bottle, add ethanol to the mark, shake well, filter, and take the filtrate to obtain the final product. [0003] This...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 陈学松韦日伟欧妮
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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