Artificial crossing breeding method of water lilies

A technology of artificial hybridization and hybridization of seeds, applied in the direction of botany equipment and methods, applications, plant gene improvement, etc., can solve problems affecting hybridization efficiency, speed up the breeding process, avoid the reduction of pollen viability, and reduce breeding work The effect of intensity

Active Publication Date: 2018-07-06
QINGDAO AGRI UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

Hybrid breeding is an effective way to cultivate new varieties of ornamental plants, but the fertilization barriers between species or varieties seriously affect the efficiency of hybridization

Method used

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  • Artificial crossing breeding method of water lilies
  • Artificial crossing breeding method of water lilies
  • Artificial crossing breeding method of water lilies

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1, water lily artificial cross breeding

[0024] Material: the water lily kind used in this embodiment has:

[0025] Tropical water lilies 'Blue Beauty', tropical water lilies 'Mrs. Martin Lenchi', tropical water lilies 'Little Brother', cold-resistant water lilies 'Ying Gem', and cold-resistant water lilies 'Rose' were all purchased from Qingdao Hongying Group.

[0026] The water lily artificial cross breeding of the present embodiment comprises the steps:

[0027] 1. Pollen collection: Choose windless weather, collect the above-mentioned tropical water lilies 'Blue Beauty', tropical water lilies 'Mrs. The anthers of four water lily varieties, '' and cold-resistant water lily 'rose', were naturally air-dried in a cool indoor place until the anthers cracked and powders were collected, and the pollen was collected for the determination of pollen viability. The method of measuring pollen viability is as follows: TTC staining method was used to measure the pol...

Embodiment 2

[0051] Embodiment 2, water lily artificial cross breeding

[0052] Material: the water lily kind used in this embodiment is identical with embodiment 1.

[0053] The water lily artificial cross breeding of the present embodiment comprises the steps:

[0054] 1. Pollen collection: Choose windless weather, collect the above-mentioned tropical water lilies 'Blue Beauty', tropical water lilies 'Mrs. The anthers of four water lily varieties, '' and cold-resistant water lily 'rose', were naturally air-dried in a cool indoor place until the anthers cracked and powders were collected, and the pollen was collected for the determination of pollen viability. The method of measuring pollen viability is as follows: TTC staining method was used to measure the pollen viability of collected pollen of 4 water lily varieties. Use a dissecting needle to evenly scatter pollen in 5mg / ml TTC (2,3,5-triphenyltetrazolium chloride) solution, cover with a cover glass, and place in a 35°C incubator fo...

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Abstract

The invention discloses an artificial crossing breeding method of water lilies. The artificial crossing breeding method of the water lilies, provided by the invention, comprises the following steps: (1) collecting pollen; (2) collecting stigma mucus; (3) germinating the pollen in the stigma mucus; (4) selecting crossing parents; (5) artificially pollinating. According to the artificial crossing breeding method of the water lilies, provided by the invention, the characteristics that the amount of the stigma mucus is great and the stigma mucus can be separated are utilized, and suitable parentsare screened through the culture of the pollen in the stigma mucus at the early period so that the huge amount of work for blindly crossing and pollinating can be avoided; the flowering phase of the water lilies is long and a phenomenon that the flowering phases of male and female parents do not meet is avoided; the male parents are bagged in the field and the pollen is collected on site in a crossing process, so that the problem that the vitality of the pollen is reduced, caused by pollen storage, is avoided. The artificial crossing breeding method of the water lilies, provided by the invention, has important meanings of alleviating the labor intensity of breeding, improving the working efficiency of breeding and accelerating a water lily new variety breeding progress.

Description

technical field [0001] The invention relates to the field of plant hybrid breeding, in particular to a water lily artificial hybrid breeding method. Background technique [0002] Water lilies are perennial aquatic perennial herbaceous flowers of the genus Nymphaea of ​​the family Nymphaeaceae. The water lily has rich colors, graceful posture and long flowering period, so it has high ornamental value. Water lilies also have many other important uses. They can absorb some toxic substances such as silver, lead, and phenol in the water body, and have the function of purifying and repairing the water body. They play a very important role in water body purification, urban greening and landscaping; The plants can be used as green manure, the rhizomes can be used as medicine, and the flowers can be used as tea. [0003] Water lilies are divided into two categories: Hardy waterlily and Tropical waterlily according to different requirements for survival temperature conditions. Cold...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 郝青刘庆超徐丽慧刘庆华王奎玲
Owner QINGDAO AGRI UNIV
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