Water Lily Artificial Hybrid Breeding Method
A technology of artificial hybridization and water lilies, which is applied in botany equipment and methods, plant gene improvement, application, etc., can solve problems affecting hybridization efficiency, speed up the breeding process, improve breeding work efficiency, and avoid the reduction of pollen viability Effect
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Embodiment 1
[0023] Embodiment 1, water lily artificial cross breeding
[0024] Material: the water lily kind used in this embodiment has:
[0025] Tropical water lilies 'Blue Beauty', tropical water lilies 'Mrs. Martin Lenchi', tropical water lilies 'Little Brother', cold-resistant water lilies 'Ying Gem', and cold-resistant water lilies 'Rose' were all purchased from Qingdao Hongying Group.
[0026] The water lily artificial cross breeding of the present embodiment comprises the steps:
[0027] 1. Pollen collection: Choose windless weather, collect the above-mentioned tropical water lilies 'Blue Beauty', tropical water lilies 'Mrs. The anthers of four water lily varieties, '' and cold-resistant water lily 'rose', were naturally air-dried in a cool indoor place until the anthers cracked and powders were collected, and the pollen was collected for the determination of pollen viability. The method of measuring pollen viability is as follows: TTC staining method was used to measure the pol...
Embodiment 2
[0051] Embodiment 2, water lily artificial cross breeding
[0052] Material: the water lily kind used in this embodiment is identical with embodiment 1.
[0053] The water lily artificial cross breeding of the present embodiment comprises the steps:
[0054] 1. Pollen collection: Choose windless weather, collect the above-mentioned tropical water lilies 'Blue Beauty', tropical water lilies 'Mrs. The anthers of four water lily varieties, '' and cold-resistant water lily 'rose', were naturally air-dried in a cool indoor place until the anthers cracked and powders were collected, and the pollen was collected for the determination of pollen viability. The method of measuring pollen viability is as follows: TTC staining method was used to measure the pollen viability of collected pollen of 4 water lily varieties. Use a dissecting needle to evenly scatter pollen in 5mg / ml TTC (2,3,5-triphenyltetrazolium chloride) solution, cover with a cover glass, and place in a 35°C incubator fo...
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