Cedarwood tissue culture fast propagation method

Abstract

The invention discloses a cedarwood tissue culture fast propagation method, which sequentially comprises the following steps of (1) explant collection and disinfection; (2) adventitious bud induction:branches subjected to disinfection treatment are subjected to trimming treatment and are then inoculated into an induction culture medium for culture until adventitious buds with the height being 1 to 3cm growing on the stem segments through induction; (3) adventitious bud multiplication: the adventitious buds are cut and taken, and are then inoculated into a multiplication culture medium for multiplication culture until the buds grow to the height being 3 to 6cm; (4) differentiation rooting: the buds are inoculated into a rooting culture medium for differentiation rooting until plants with the length being greater than or equal to 4cm and at least three roots with the length being greater than 2cm are obtained; (5) performing seedling hardening and transplanting on the obtained plants. The method has the advantages that excellent clonal stem segments are used as explants; a large number of cedarwood tissue culture seedlings can be fast obtained by the method.

Description

technical field [0001] The invention relates to a rapid propagation method of Chinese fir tissue culture. Background technique [0002] Chinese fir (Cunninghamia lanceolate) is one of the unique tree species in my country. It also plays an important role in China's artificial forestry. It is mainly distributed in the Yangtze River Basin and south of the Qinling Mountains in China. It grows fast, the trunk is straight and round, and the wood texture is beautiful. Uniform structure, excellent material, less pests and diseases and high wood yield, therefore, it is also widely used as economic forest tree species. The development of Chinese fir forests is still relatively slow. At present, Chinese fir mainly adopts traditional methods such as cuttings, grafting and sowing to raise seedlings. There are disadvantages such as high seedling cost, poor growth of seedlings, uneven growth, and failure to fully develop the potential of improved species. The supply of high-quality strong...

AI Technical Summary

Problems solved by technology

The development of Chinese fir forests is still relatively slow. At present, Chinese fir mainly adopts traditional methods such as cuttings, grafting and sowing to raise seedlings. There are disadvantages such as high seedling cost, poor growth of seedlings, uneven growth, and failure to fully develop the potential of improved species. The supply of high-quality strong seedlings cannot meet the demand of the market

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