Genetic typing detection kit used for 23 genetic site of human Rh blood type

Abstract

The invention belongs to the technical field of human nucleic acid in vitro detection, in particular to a genetic typing detection kit for 23 genetic site on human No.5, No.19, No.21 and X / Y chromosomes. The genetic typing detection kit is characterized in that: a composite PCR (Polymerase Chain Reaction) amplification system for performing genetic typing on 23 genetic site distributed on the human No.5, No.19, No.21 and X / Y chromosomes is provided; PCR amplification primer group is shown as SEQ ID No.1- SEQ ID No.53; the genetic typing detection kit comprises a primer composition container and a PCR reaction mother liquor container; the primer composition container comprises a primer SEQ ID No.1- SEQ ID No.53 composition stock solution. By adopting genetic typing detection kit, the single-tube amplification for 23 genetic site, genetic site amplification balance in each fluorescent channel and different fluorescent channels, good typing spectrum are realized, the cost, labor force andtime are saved remarkably, and the working efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of in vitro detection of human nucleic acid, and specifically relates to the detection of the genotype of a polymorphic gene site in human genome DNA, and specifically relates to a method for detecting human No. 5, No. 19, and No. A kit for Rh blood group genotyping detection for 23 gene loci on the No. and X / Y chromosomes. Background technique [0002] According to the presence or absence of Rh factor, Rh negative and Rh positive blood types can be distinguished. This blood group system is called Rh blood group system. Rh is the first two letters of the foreign language name of the rhesus monkey (Rhesus Macacus). In 1940, when scientists such as Landsteiner conducted animal experiments, they found that Rh blood group antigens existed on the red blood cells of rhesus monkeys and most human beings, so it was named. Those who have Rh agglutinogen on red blood cells of human blood are Rh positive. Otherwise ...

AI Technical Summary

Problems solved by technology

Due to the lack of mature detection methods in China, there is still a lack of systematic research on the molecular background and population distribution of the Rh blood group in the domestic population. However, some studies have pointed out that the genetic mechanism of the Rh blood group in China is diverse and complex. Studies in different regions of China have found that China is Rh(D) negative. Only about 50% of the population is caused by the deletion of the RhD gene, about 20-30% are the diffuse type, of which more than 95% are 1227G>A mutations, and the remaining about 10-20% are weak D phenotypes, of which 60% The above is weak D15 type in weak D, more than 30% is DVI Type3 type in part D

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