Method for detecting gas forming microorganisms contaminated in milk

A technology of microorganisms and microbial counts, applied in the field of microorganisms, can solve the problems of inability to quickly detect gas-producing microorganisms, qualitative and quantitative characteristics of gas-producing microorganisms, etc., and achieve the effects of low cost, simple operation, and broad application prospects

Active Publication Date: 2018-09-25
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problems in the prior art that the gas-producing microorganisms contaminated in milk cannot be quickly detected, and the gas-producing microorganisms contaminated in milk cannot be quickly qualitatively and quantitatively determined, the present invention provides a method for detecting the gas-producing microorganisms contaminated in milk.

Method used

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  • Method for detecting gas forming microorganisms contaminated in milk

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Effect test

Embodiment 1

[0024] Embodiment one: a kind of method that the present invention provides to detect the contaminated gas-producing microorganisms in milk, comprises the following steps:

[0025] (1) Inoculate various gas-producing microorganisms into culture media suitable for their own gas-producing microorganisms, and record the gas production of various gas-producing microorganisms at different times;

[0026] (2) Add the milk samples to be tested in the test tubes of various culture media respectively, and the culture media are the culture media before inoculation in step (1), observe the gas production situation in the test tubes containing various culture media, according to the steps ( 1) The recorded gas production of various gas-producing microorganisms at different times can determine the type of gas-producing microorganisms contaminated in the milk.

[0027] In the present invention, when observing the gas production in the test tubes containing various culture media in the step ...

Embodiment 2

[0037] Embodiment two: a kind of method that the present invention provides detects the contaminated gas-producing microorganism in milk, comprises the following steps:

[0038] (1) Inoculate various gas-producing microorganisms into culture media suitable for their own gas-producing microorganisms, and record the gas production of various gas-producing microorganisms at different times;

[0039] (2) Add the milk samples to be tested in the test tubes of various culture media respectively, and the culture media are the culture media before inoculation in step (1), observe the gas production situation in the test tubes containing various culture media, according to the steps ( 1) The recorded gas production of various gas-producing microorganisms at different times can determine the type of gas-producing microorganisms contaminated in the milk.

[0040] In the present invention, when observing the gas production in the test tubes containing various culture media in the step (2)...

Embodiment 3

[0056] Embodiment 3: This embodiment uses the method of the present invention to detect the types and counts of gas-producing microorganisms in liquid milk systems (pasteurized milk, ultra-high temperature sterilized milk, i.e. UHT milk):

[0057] Inoculate Escherichia coli into two milk systems: pasteurized milk as sample A and UHT milk as sample B;

[0058] Inoculate the yeast into two milk systems: pasteurized milk as sample C and UHT milk as sample D;

[0059] (1) Strain activation

[0060] The initial bacterial liquid was obtained as in Example 2, and the two bacteria were separately inoculated in the two milk systems in different proportions.

[0061] (2) The sample dilution step is as in Example 1

[0062] (3) Inoculation

[0063] Inoculate the diluted 3 concentration gradient samples into test tubes (10% inoculum size) of the appropriate medium for the corresponding bacterial strains, do 3 parallel experiments for each dilution gradient, control the inoculation time...

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Abstract

The invention discloses a method for detecting gas forming microorganisms contaminated in milk. The method comprises the following steps: (1) inoculating various gas forming microorganisms into suitable media for cultivating, and recording various gas forming conditions of the gas forming microorganisms at different times; (2) separately adding milk samples to be tested into test tubes containingvarious culture medium, the culture medium are culture medium before the inoculation in the step (1), and observing the gas forming conditions in the test tubes containing the various media, wherein according to the gas forming conditions of the various gas forming microorganisms recorded in the step (1) at different times, the types of the gas forming microorganisms in the milk samples to be tested can be determined. The method has the advantages that the types of gas forming microorganisms in milk samples to be tested can be judged within 48 hours and counted, so as to quickly check the sources and the causes of pollution, determine microorganisms causing swelling, and take effective measures in time to control and reduce loss.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a method for detecting gas-producing microorganisms contaminated in milk. Background technique [0002] Dairy products may bulge during transportation and storage. It is easy to judge the bulge caused by physical factors, but it is difficult to judge the bulge caused by microbial factors, and it is also difficult to judge the possible presence of bacteria. Quantification of gas-producing microorganisms. [0003] There are two types of gas-producing microorganisms commonly found in food, including dairy products, including yeast and bacteria. In order to detect the microorganisms that cause the packaging of dairy products to bulge, the existing technology needs to culture the contaminated microorganisms in the milk on a plate, isolate a single colony and then cultivate them, and then perform qualitative detection to know which microorganisms (yeast, bacteria) ) to produce...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12Q1/04C12R1/19C12R1/645
CPCC12Q1/04C12Q1/10G01N2333/39G01N2333/245
Inventor 游春苹徐明亮刘振民吴正钧张红发李楠鄢明辉扶晓菲
Owner BRIGHT DAIRY & FOOD
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