Microsatellite Polymorphic Sites, Identification Methods and Specific Primer Sequences of Crested Ibis

Abstract

The invention provides crested ibis microsatellite polymorphic sites, an identification method and specific primer sequences. 12 pairs of crested ibis microsatellite polymorphism site specific primersequences are screened, and the sequences are shown as SEQ ID NO. 1-SEQ ID NO. 24. The identification method of the crested ibis microsatellite polymorphic sites is used for screening the microsatellite sites and designing high quality primers; read data of crested ibis genome sequencing is compared to a crested ibis reference genome to identify the INDEL sites; according to the INDEL sites and microsatellite site information, the polymorphic microsatellite sites and high quality primers are screened. The 12 pairs of microsatellite primers are all polymorphic sites and have strong resolving power. Using the set of microsatellite primers can carry out individual identification and parent-child relationship identification of the crested ibis economically, rapidly and correctly, be beneficialfor family lineage reconstruction and information improvement of crested ibis population, and provide data support for the formulation of a scientific crested ibis protection program.

Description

technical field [0001] The invention belongs to the field of crested ibise microsatellite polymorphic site identification, and in particular relates to the crested ibise microsatellite polymorphic site, an identification method and a specific primer sequence. Background technique [0002] Microsatellites refer to DNA short tandem repeats (simple sequence repeat, SSR), which consist of a core sequence and flanking sequences on both sides. There are 2-6 nucleotides in the core sequence room. Usually, the repeat number of the microsatellite core sequence has a strong positive correlation with the number of alleles at this site. The larger the core sequence repeat number, the greater its variability, and the The number of alleles of microsatellite micropoint is more. Microsatellites have the characteristics of large number, uniform distribution in the genome, rich polymorphism information, and easy detection. Co-dominant allele analysis can be done on a single microsatellite lo...

AI Technical Summary

Problems solved by technology

The previous method of screening microsatellites using species reference genomes has two defects: 1. The specificity of primer design is poor, which is prone to non-specific amplification; 2. The polymorphism of the screening sites is low, and the follow-up costs Much time and cost for further screening

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