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Genotype sequences for HLA typing

A genotype and sequence technology, applied in the field of HLA typing, can solve the problems of long detection time and low efficiency, and achieve the effect of improving typing accuracy and improving treatment efficiency.

Active Publication Date: 2018-10-09
BGI GENOMICS CO LTD
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Problems solved by technology

The existing technology needs to detect the two haplotypes separately, which takes a long time and is inefficient

Method used

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  • Genotype sequences for HLA typing
  • Genotype sequences for HLA typing
  • Genotype sequences for HLA typing

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Embodiment Construction

[0029] In the embodiment of the present invention, in combination with the information analysis method, one of the haplotypes is determined first, and then the sequence of the other haplotype is determined using experimental techniques. Compared with the previous method, this method can save half the time and improve the efficiency by 50%. Based on this method, some new gene sequences were discovered, and the contributions to HLA-A, -B, -C, DRB1, and DQB1 in the current database were 0.82%, 0.67%, 0.91%, 1.52%, and 3.07%, respectively. The embodiment of the present invention shows 10 new genotype sequences.

[0030] The method flowchart of the embodiment of the present invention is as follows figure 1 As shown, it generally includes the following steps:

[0031] The sample source is the DNA library of the bone marrow or umbilical cord blood sample, which is placed in a sequencer for sequencing;

[0032] HLA typing analysis was performed on the sequencing data using the RCHS...

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Abstract

The invention provides a set of genotype sequences for HLA typing. The genotype sequences comprise a nucleotide sequence as shown in SEQ ID No. 1, and optionally comprise at least one of the nucleotide sequences as shown in SEQ ID No. 2-10. The genotype sequences for HLA typing of the invention enrich a HLA database, and can effectively improve typing accuracy, thereby improving the treatment efficiency of related diseases, such as thalassemia, acute leukemia and bone marrow transplantation.

Description

technical field [0001] The invention relates to the technical field of HLA (human leukocyte antigen, human leukocyte antigen) typing, in particular to a set of genotype sequences for HLA typing. Background technique [0002] From the 1970s to the 1980s, HLA typing techniques were mainly serology and cytology. Since the 1990s, the development of molecular biology, especially the introduction of PCR technology, has made molecular technology widely used in clinical diagnosis, and HLA typing is no exception (WoszczekG, et al. "Comparison of serological and molecular (PCR- SSP) techniques of HLA-DR typing in clinical laboratory routine". Ann Transplant. 1997; 2(1):39-42). The DNA typing method of HLA was proposed at the 11th International HLA Symposium in 1991. With the rapid development of sequencing technology, the typing method based on DNA sequence has replaced the traditional serological and cytological typing methods. At present, DNA typing methods are mainly divided into...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6881C12N15/11
CPCC12Q1/6881C12Q2600/156
Inventor 柴相花杨晓琴方俊彬杨帆陈琳袁玉英张红云刘娜尹烨
Owner BGI GENOMICS CO LTD
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