Renibacterium salmoninarum strain QD-01 efficiently producing chitosanase as well as fermentation method and application thereof

Abstract

The invention provides a renibacterium salmoninarum strain QD-01 capable of efficiently producing chitosanase as well as a fermentation method and application thereof. According to the invention, fermentation conditions of screened strains are optimized, including single-factor condition optimization and orthogonal experiment optimization of chitosanase production, and an optimal enzyme productioncondition is obtained. The adaptive temperature range of the chitosanase produced by the strain is 35-60 DEG C, and the pH value is 4.0-9.0; and the enzyme activities of a fermentation broth of the chitosanase and freeze-dried crude enzyme powder are compared, and the result shows that the enzyme activity of the crude enzyme powder is basically unchanged, so the preparation could be freeze-driedinto powder for storage and transportation. According to the invention, the chitosanase produced by fermentation is adopted to explore a preparation process of chitosan oligosaccharide, thus shortening the preparation period of chitosan oligosaccharide; moreover, the chitosanase has high specificity. The strain provided by the invention and the prepared chitosanase have wide market application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a high-efficiency chitosanase-producing Nephrobacterium salmoninae strain QD-01 and a fermentation method and application thereof. Background technique [0002] At present, the biological method is to use chitosan enzyme to degrade chitosan production process to gradually replace the traditional chemical method, which has the advantages of high degradation efficiency, relatively uniform products, and little environmental pollution; chitosan enzyme preparations on the market now exist The purity is small, the enzyme activity is not high, and the storage period is short. Therefore, people continue to study high-efficiency chitosanase in the hope of improving the production process and increasing production efficiency. [0003] Most of the bacteria and fungi that can produce chitosanase have the disadvantage of low enzyme production. People hope to improve the productio...

AI Technical Summary

Problems solved by technology

[0002] At present, the biological method is to use chitosan enzyme to degrade chitosan production process to gradually replace the traditional chemical method, which has the advantages of high degradation efficiency, relatively uniform products, and little environmental pollution; chitosan enzyme preparations on the market now exist The purity is small, the enzyme activity is not high, and the storage period is short. Therefore, people continue to study high-efficiency chitosanase in the hope of improving the production process and increasing production efficiency.

[0003] Most of the bacteria and fungi that can produce chitosanase have the disadvantage of low enzyme production. People hope to improve the production of chitosanase by gene mutation, engineering bacteria transformation, etc. Low efficiency, long cycle and other disadvantages are not conducive to industrial production

Images