A high-efficiency chitosanase-producing Nephrobacter salmoninae strain qd-01 and its fermentation method and application

Abstract

The invention provides a high-efficiency chitosanase-producing Renobacter salmoninae strain QD-01 and its fermentation method and application. The present invention conducts optimization research on fermentation conditions for the screened bacterial strains, including optimization of single factor conditions and orthogonal experiment optimization for chitosanase production, and obtains the best enzyme production conditions. The adaptable temperature range of the chitosanase produced by the strain is 35-60°C; the pH is 4.0-9.0; the enzyme activity of the chitosanase fermentation liquid and the freeze-dried crude enzyme powder were compared, and it was found that the crude enzyme powder The enzyme activity is basically unchanged, so the preparation can be freeze-dried into powder, which is convenient for storage and transportation. The invention uses the chitosanase produced by fermentation to explore the chitosan oligosaccharide preparation process, shortens the preparation cycle of the chitosan oligosaccharide; and the chitosanase has high specificity. The bacterial strain provided by the invention and the prepared chitosanase have broad market application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a high-efficiency chitosanase-producing Nephrobacterium salmoninae strain QD-01 and a fermentation method and application thereof. Background technique [0002] At present, the biological method is to use chitosan enzyme to degrade chitosan production process to gradually replace the traditional chemical method, which has the advantages of high degradation efficiency, relatively uniform products, and little environmental pollution; chitosan enzyme preparations on the market now exist The purity is small, the enzyme activity is not high, and the storage period is short. Therefore, people continue to study high-efficiency chitosanase in the hope of improving the production process and increasing production efficiency. [0003] Most of the bacteria and fungi that can produce chitosanase have the disadvantage of low enzyme production. People hope to improve the productio...

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Problems solved by technology

[0002] At present, the biological method is to use chitosan enzyme to degrade chitosan production process to gradually replace the traditional chemical method, which has the advantages of high degradation efficiency, relatively uniform products, and little environmental pollution; chitosan enzyme preparations on the market now exist The purity is small, the enzyme activity is not high, and the storage period is short. Therefore, people continue to study high-efficiency chitosanase in the hope of improving the production process and increasing production efficiency.

[0003] Most of the bacteria and fungi that can produce chitosanase have the disadvantage of low enzyme production. People hope to improve the production of chitosanase by gene mutation, engineering bacteria transformation, etc. Low efficiency, long cycle and other disadvantages are not conducive to industrial production

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