Immunoregulatory polynucleotide and application thereof

一种多核苷酸、调节性的技术，应用在有机活性成分、含有效成分的医用配制品、动物/人类蛋白质等方向，能够解决难以达到长期有效地抑制和杀伤肿瘤细胞等问题

Inactive Publication Date: 2018-11-02
CHANGCHUN HUAPU BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems that need further research to be solved, such as antibody immunogenicity, drug resistance of long-term use of tumor targets, and long-term effects of simple single blockade of signal transduction pathways
In short, more than half of the antibodies are difficult to achieve long-term effective inhibition and killing of tumor cells

Method used

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  • Immunoregulatory polynucleotide and application thereof
  • Immunoregulatory polynucleotide and application thereof
  • Immunoregulatory polynucleotide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0329] Materials and methods

[0330] CpG oligonucleotide (CpG-ODN): CpG-ODN was synthesized by Suzhou Ruibo Biotechnology Co., Ltd. CpG 1-12 are modified sequences. Type A CpG2216 (5'-ggGGGACGATCGTCgggggG-3'SEQ ID NO.:13), Type B CpG2006 (5'-TCGTCGTTTTGTCGTTTTGTCGTT-3, SEQ ID NO.:14) and CpG 684 (5'-TCgACgTTCgTCgTTCgTCgTTC-3', SEQ ID NO.:14) ID NO.:15), CpG 1018 (5'-TGACTGTGAACGTTCGAGATGA-3', SEQ ID NO.:16) and C-type CpG2395 (5'tcgtcgttttcggcgcgcgccg-3', SEQ ID NO.:17) were used as positive controls, respectively. GC-ODN (5'-tggccaagcttgggccccttgcaagggcc-3'SEQ ID No.: 18) was used as a negative control. All CpG-ODNs were dissolved in sterile / endotoxin-free water (InvivoGen, USA) and stored at -40°C for future use.

[0331] Human peripheral blood leukocytes and experimental animals:

[0332] Human peripheral blood leukocytes were purchased from Changchun Central Blood Station.

[0333] Mice and cell lines. Female BALB / c mice (6-8 weeks old) were purchased from ...

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Abstract

The invention relates to a composition containing immunoregulatory polynucleotide serving as a therapeutic agent (such as immunization therapy). Further, the invention relates to application of the composition to treatment of diseases such as cancer.

Description

technical field [0001] The present invention relates to compositions comprising immunomodulatory polynucleotides as therapeutic agents, eg, for immunotherapy. Further, the invention relates to the use of said composition in the treatment of diseases such as cancer. Background technique [0002] The ability of various TLR agonists to promote anti-tumor immunity has been investigated in clinical trials. Antitumor responses are largely due to their ability to stimulate APCs, such as dendritic cells (DCs), which in turn activate tumor-specific T cell responses. [0003] Toll-like receptor 9 (TLR9) refers to unmethylated CpG dinucleotides, which are hallmarks of microbial DNA, which can be synthesized by synthetic oligonucleotides containing CpG motifs. Nucleotide (CpG ODN) simulation. TLR9 stimulation by CpG DNA or CpG ODN triggers intracellular signaling that activates macrophages, DC cells and B cells and produces cytokines, chemokines and immunoglobulins. Subsequently, cy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/117A61K31/711A61K45/06A61P35/00
CPCA61K31/711A61K45/06C12N15/117A61P35/00C12N2310/17C12N2310/315C12N2320/31A61K31/05A61K2300/00C07K14/57A61K48/00A61K31/7115C12N15/111
Inventor 黄涛邵彦
Owner CHANGCHUN HUAPU BIOTECHNOLOGY CO LTD
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