A gene set for liver cancer detection and its panel detection design method

Abstract

The invention discloses a method for preparing a liver cancer gene detection panel, comprising the following steps: screening liver cancer information and related genes to obtain a gene set; selecting a gene target region; designing a panel probe; synthesizing an RNA single-stranded probe to obtain a gene detection panel. The gene detection panel designed by the present invention can be used for the diagnosis, typing and prognosis of liver cancer, has wide gene coverage and strong portability, and is suitable for newly developed detection platforms.

Description

technical field [0001] The invention belongs to the field of gene detection, in particular to a gene set used for liver cancer detection and a panel detection design method thereof, and also relates to the application of the gene set in a gene chip. Background technique [0002] Liver cancer is one of the most common malignant tumors in clinical practice. According to statistics, there are about 740,000 new liver cancer cases and 700,000 liver cancer deaths worldwide each year, about half of which occur in China. Due to the high morbidity and high mortality of liver cancer, liver cancer is paid special attention in the world, and the incidence and mortality of liver cancer in my country are also increasing year by year. It seriously endangers human health and has become a global and serious public health problem. The occurrence and development of liver cancer is a complex biological process. In addition to the patient's own susceptibility to genetic factors, various extrace...

AI Technical Summary

Problems solved by technology

The technology of diagnosing liver cancer by high-throughput sequencing of the genome in biological samples has made great progress, but the existing liver cancer detection has the following defects: 1. The detection content is single and the function is limited

At present, most panel designs are only aimed at drug guidelines, labels, and limited literature collection, and there are few comprehensive analyzes based on the results of large-scale sequencing platforms, public databases, and text mining techniques

3. At present, the technical process of panel design and synthesis method is single, and there are always areas that cannot be designed according to the fixed algorithm, so it is inevitable to omit some important sites

4. There are few applicable platforms. For the developed panel detection, there is already a set of detection system schemes on common platforms, but for new detection platforms, such as BGISEQ-500, there is no suitable detection scheme yet

5. The process of information analysis method is cumbersome

The bioinformatics analysis of off-machine data needs to be evaluated step by step, and the results are completed step by step. The steps are cumbersome, time-consuming, and the portability is not high, which cannot meet the current analysis needs.

Images