A method for separating and enriching platelets, a drug testing method for platelet-acting drugs, and a drug testing chip

Abstract

The invention discloses a platelet separation and enrichment method, a platelet effect drug trial method and a platelet effect drug trial chip, and relates to the field of platelet detection. The platelet separation and enrichment method is as follows: allowing a blood sample to flow in a pipe, applying a dielectrophoretic force; adding an inducer to platelets; allowing the mixture to flow in thepipe, applying a dielectrophoretic force, and detecting amounts of aggregated platelets and platelets. The platelet separation and enrichment method can quickly and effectively separate the plateletsfrom the blood sample. The platelet effect drug trial method is as follows: adding a platelet effect drug to the mixture; allowing the mixture after the action of the drug to flow in the pipe, applying a dielectrophoretic force, and detecting the amounts of aggregated platelets and platelets, and the platelet effect drug trial method can quickly detect changes in aggregation behaviors of the platelets after the action of the drug. The platelet effect drug trial chip has a simple structure and a low cost, and can quickly perform a platelet effect drug trial.

Description

technical field [0001] The invention relates to the field of platelet detection, and in particular to a platelet separation and enrichment method, a test method for platelet-acting drugs and a test chip. Background technique [0002] As an important component of peripheral blood, platelets participate in many physiological and pathological processes in the body. During the physiological process of blood coagulation, the extracellular matrix protein at the vascular injury is exposed to the blood, and a small amount of platelets will first adhere to the surface of the extracellular matrix protein, and then the platelets will be activated, through autocrine adenosine diphosphate (ADP) and thromboxane A2 (TXA2) and other coagulation factors recruit other platelets from the blood, and finally aggregate through the combination of platelet GPⅡb / Ⅲa receptors and fibrinogen. Inhibition of platelet adhesion and aggregation will lead to bleeding risk, while hyperfunction will increase...

AI Technical Summary

Problems solved by technology

However, the above-mentioned existing detection technologies and systems are very complicated and costly, and are only suitable for professional testing departments, and obviously not suitable for rapid drug testing.

In addition, in order to quickly test the drug, it is necessary to quickly separate the platelets in the blood of individual patients, but the existing platelet separation methods and equipment also have the defects of complexity, high cost, and easy destruction of platelets.

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