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Method for determining salmeterol, indacaterol and olodaterol in pork

A pork and muscle technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of difficulty in rapid analysis and detection, complicated operation steps, and high detection cost, and achieve the effects of high sensitivity, high ion response value, and accurate quantification.

Inactive Publication Date: 2018-12-11
食药环检验研究院(山东)集团有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

However, due to the different physical and chemical properties of veterinary drugs, and the interference of other components in the test samples, it is very difficult to quickly analyze and detect salmeterol, indacaterol, and olodaterol residues in animal tissues.
There are currently no national standard methods for salmeterol, indacaterol, and olodaterol. The methods in the literature also need to use multiple different methods to detect these compounds. The operation steps are complicated and the detection cost is high. Therefore, it is urgent to develop a A general detection method for the detection of routine samples

Method used

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  • Method for determining salmeterol, indacaterol and olodaterol in pork

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Embodiment 1

[0049] A method for measuring salmeterol, indacaterol, olodaterol, and three kinds of beta-receptor agonists in pork, the method is to use liquid chromatography-mass spectrometry to detect pork, comprising the following steps:

[0050] S1 sample preparation

[0051] Take the edible muscle sample of the tested pork and cut it into 2cm×2cm small pieces (remove the skin, bones, viscera, etc.), put it in a meat grinder and chew it, and store the prepared sample at -16°C;

[0052] S2 extraction

[0053](1) Weigh 2.00 g of the crushed sample into a 50 mL centrifuge tube, add 8 mL of sodium acetate buffer and mix thoroughly, then add 50 μL of β-glucuronidase, mix well, and hydrolyze in a 37°C water bath for 12 hours;

[0054] (2) Cover the sample to be tested and shake for 15 minutes, centrifuge for 5 minutes (8000r / min), take 4mL supernatant in a centrifuge tube, add 5mL of 0.1mol / L perchloric acid solution, cover and shake for 5~10min, put all the supernatant The supernatant (abo...

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Abstract

The invention provides a method for determining salmeterol, indacaterol and olodaterol in pork. The method includes the following steps: step 1, preparing samples; step 2, extracting to-be-detected samples; step 3, determining detection conditions of a liquid chromatography-tandem mass spectrometer and performing detection; step 4, drawing standard working curves; step 5, according to liquid chromatograms of to-be-detected solutions in the S2, calculating the contents of the olodaterol, the indacaterol and the salmeterol in the pork. The method has the advantages that the salmeterol, the indacaterol and the olodaterol in the pork are simultaneously determined for the first time, and the blank in current detection of the salmeterol, the indacaterol and the olodaterol in the pork is filled up; ion response values are high, sensitivity is high, detection limits are low, qualitativeness, quantitativeness, accuracy, rapidness, high efficiency and sensitivity are truly realized, and the method can serve as a reliable measure to detect the three drugs in the pork.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a method for determining salmeterol, indacaterol and olodaterol in pork. Background technique [0002] β2 receptor agonists can relax airway smooth muscle, reduce mast cell and basophil degranulation and the release of mediators, reduce microvascular permeability, increase The swinging of airway epithelial cilia etc. relieves asthma symptoms. There are many such drugs, which can be divided into short-acting (maintaining effect for 4-6 hours) and long-acting (maintaining for 12 hours) β2 receptor agonists, among which salmeterol, indacaterol, and olodaterol are common varieties . [0003] Unreasonable use of salmeterol, indacaterol, and olodaterol may easily cause drug residues, and at the same time, residual drugs may cause potential harm to human health, so the drug must be stopped on time to ensure the quality and safety of animal products. Monitoring and ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 孙学文陈艾亭
Owner 食药环检验研究院(山东)集团有限公司
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