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Method for simultaneously measuring contents of three fat-soluble vitamins in multiple vitamin injections

A technology for fat-soluble vitamins and vitamins, applied in the field of analytical chemistry, can solve the problems of gradient elution, complex mobile phase, cumbersome and time-consuming sample processing, etc., and achieve the effects of accurate determination, labor saving and improvement of detection efficiency

Active Publication Date: 2018-12-28
河北远征药业有限公司
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for simultaneously measuring the contents of three fat-soluble vitamins in multivitamin injections. The problem of tedious and time-consuming sample processing

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  • Method for simultaneously measuring contents of three fat-soluble vitamins in multiple vitamin injections
  • Method for simultaneously measuring contents of three fat-soluble vitamins in multiple vitamin injections
  • Method for simultaneously measuring contents of three fat-soluble vitamins in multiple vitamin injections

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Embodiment Construction

[0024] The technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do it without departing from the meaning of the present invention. By analogy, the present invention is therefore not limited to the specific examples disclosed below.

[0026] This embodiment ...

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Abstract

The invention discloses a method for simultaneously measuring contents of three fat-soluble vitamins in multiple vitamin injections, and relates to the field of analytic chemistry. The method in the invention comprises the steps of: taking octadecylsilane chemically bonded silica as the stationary phase, and filling in the chromatographic column of a liquid chromatograph; preparing methanol-acetonitrile-ethyl acetate as the mobile phase; precisely weighing proper vitamin A, vitamin D3 and vitamin E contrasts, and diluting by adding isopropyl alcohol to obtain contrast solution; precisely weighing multiple vitamin injections to be measured, and diluting by using isopropyl alcohol to prepare test solution; respectively taking contrast solution and test solution, injecting into the liquid chromatograph to perform isocratic elution, and recording the chromatogram; and calculating the concentration of the test solution in terms of peak area according to the external standard method. By means of the method in the invention, the problems that gradient elution and the mobile phase are complex and sample processing is fussy and time-saving due to the fact that three different detection wavelengths are adopted in the prior art are solved.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for simultaneously determining the contents of three fat-soluble vitamins in multivitamin injections. Background technique [0002] Vitamins are a class of organic compounds necessary to maintain human health, promote growth and development, and regulate physiological functions. With the development of society, more and more multivitamin products have appeared on the market. Then, the determination method of vitamin in the product is particularly important. [0003] vitamin A, vitamin D 3 Vitamin E and vitamin E are fat-soluble vitamins, which are easy to accumulate in the body. Excessive intake will lead to poisoning. This requires a rapid detection method for fat-soluble vitamins. Now, high-performance liquid chromatography is more commonly used. The detection of vitamins A, D, and E in food, dairy products, serum, and injections has been reported, but there are ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 宋婷婷刘毅刘静瞿红颖郭建立魏永辉郭鸿志李红园魏丽娟
Owner 河北远征药业有限公司
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