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A kind of SNP molecular marker related to oestrous traits of sheep, its detection kit and application

A molecular marker and kit technology, which is used in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., to achieve the effect of improving the estrus breeding rate

Active Publication Date: 2021-06-08
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

However, there are no specific DNA molecular markers that can be used for the screening of oestrus traits in sheep

Method used

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  • A kind of SNP molecular marker related to oestrous traits of sheep, its detection kit and application
  • A kind of SNP molecular marker related to oestrous traits of sheep, its detection kit and application
  • A kind of SNP molecular marker related to oestrous traits of sheep, its detection kit and application

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Embodiment 1

[0075] Identification of SNP Molecular Markers Associated with Seasonal Estrus in Sheep

[0076] Through whole-genome resequencing of 99 sheep individuals from 10 sheep breeds, including three breeds of Tibetan sheep (valley-type Tibetan sheep, grassland-type Tibetan sheep, and Euler sheep), and 6 Mongolian sheep (small-tailed Han sheep, Wu Everest, Tan sheep, Hu sheep, Cele black sheep, and Bayinbulak) and 1 European sheep (Australian Merino), obtained the largest number of genetic polymorphic sites in the sheep genome so far, and analyzed these sheep The genetic diversity and population genetic structure of the population were screened for genes related to seasonal estrus. And through the calculation of Fst value, a large number of effective SNP sites were obtained and some genes were screened, including the SNP sites of the FBXL3 gene related to sheep seasonal estrus. It was found that the FBXL3 gene located at the 52551820bp site of chromosome 10 (NC_019467.2, based on th...

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Abstract

The invention provides a SNP molecular marker related to sheep estrus traits and its detection kit and application, and belongs to the technical field of sheep SNP molecular markers. The SNP site is located at No. 52551820bp of sheep No. Sheep genome sequence information version number Oar_v4.0, December 2015), there is a G / A base mutation at the SNP site, which has a significant correlation with the seasonal estrus traits of sheep. The estrous character of the sheep to be tested can be determined by typing the SNP site. The method for detecting the genotype of the SNP locus of sheep provided by the present invention has higher sensitivity, higher accuracy, and higher cost performance, and can realize automatic detection of the SNP locus. The AA homozygous individuals and GA heterozygous individuals are selected to increase the estrous mating rate of sheep, which has potential application value for large-scale molecular breeding of sheep.

Description

technical field [0001] The invention belongs to the technical field of sheep SNP molecular markers, in particular to a SNP molecular marker related to sheep estrus traits, a detection kit and application thereof. Background technique [0002] Single nucleotide polymorphism (SNP) mainly refers to the polymorphism of DNA fragments caused by the variation of a single deoxynucleotide on the genome deoxynucleic acid (DNA) sequence. The polymorphism of SNP only involves the variation of a single base, and the expression forms include substitution, insertion and deletion. SNP detection methods currently commonly used include Sanger sequencing, DNA chips, time-of-flight mass spectrometry, and the latest high-throughput next-generation sequencing technologies. Genotype detection by detecting single nucleotide polymorphism markers is a genotype detection method that has emerged in recent years. As a genetic marker, SNP has been widely used in research fields such as gene mapping, cl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 储明星刘秋月贺小云狄冉王翔宇胡文萍
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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