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Application of nitrogen-fixing anabaena to promote growth of mulberry tree and increase yield of mulberry leaves

An anabaena, nitrogen fixation technology, applied in the agricultural field, can solve the problems of high price, complicated preparation process and high cost, and achieve the effect of increasing yield

Inactive Publication Date: 2019-01-18
卢梅雅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In this preparation method, not only need to use expensive cellulase and protease, the cost is high, and the whole preparation process is relatively complicated

Method used

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  • Application of nitrogen-fixing anabaena to promote growth of mulberry tree and increase yield of mulberry leaves
  • Application of nitrogen-fixing anabaena to promote growth of mulberry tree and increase yield of mulberry leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Nitrogen-fixing Anabaena 119 (provided by Wuhan Institute of Hydrobiology, Chinese Academy of Sciences) was inoculated in BG11 nitrogen-deficient medium, and cultivated at 28°C, 150r / min, light of 60000-80000lx, and light-dark cycle of 12h-12h. After 7 days, the seed solution was obtained; then the seed solution was poured into the small pool set up in the greenhouse for large-scale cultivation, and the temperature in the greenhouse was kept at about 30-35°C; after 5 days of expansion cultivation, the nitrogen-fixing Anabaena can be collected , press filtration to remove most of the water to obtain wet algae with a water content of 40-60%; then spread the wet algae on a tray and place it in an oven at 35°C until the water content drops to 8-10% to obtain dry algae. algae; after cooling, crush the dried algae with a pulverizer; pack the crushed dry algae in 5L conical flasks, pour into 90% ethanol aqueous solution for leaching, dry algae and ethanol The mass-to-volume ra...

Embodiment 2

[0031] Nitrogen-fixing Anabaena 119 (provided by Wuhan Institute of Hydrobiology, Chinese Academy of Sciences) was inoculated in BG11 nitrogen-deficient medium, and cultivated at 28°C, 150r / min, light of 60000-80000lx, and light-dark cycle of 12h-12h. After 7 days, the seed solution was obtained; then the seed solution was poured into the small pool set up in the greenhouse for large-scale cultivation, and the temperature in the greenhouse was kept at about 30-35°C; after 5 days of expansion cultivation, the nitrogen-fixing Anabaena can be collected , press filtration to remove most of the water to obtain wet algae with a water content of 40-60%; then spread the wet algae on a tray and place it in an oven at 40°C until the water content drops to 8-10% to obtain dry algae. algae; after cooling, crush the dried algae with a pulverizer; pack the crushed dry algae in a 5L conical flask, pour into an aqueous ethanol solution with a volume fraction of 95%, and extract the dried algae...

Embodiment 3

[0033] Set up 24 test areas in the mulberry garden, the area of ​​each test area is 20 square meters, and each test area is selected in the central position of the mulberry garden, so that the strain number and size of the mulberry trees in each test area are basically the same, avoiding the mulberry garden. Effect of marginal region specificity on assay.

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Abstract

The invention discloses application of nitrogen-fixing anabaena to promote growth of mulberry trees and increase yield of mulberry leaves. The application comprises: collecting nitrogen-fixing anabaena, adjusting water content of the nitrogen-fixing anabaena to less than 15%, and pulverizing; and performing digestion on the pulverized nitrogen-fixing anabaena in ethanol to obtain an extract liquidand residues respectively; concentrating and drying the extract liquid, to obtain dry powders, dissolving the dry powders in water to obtain a spray liquid, and spraying the spray liquid on mulberrybranches and leaves; applying the residues in mulberry root soil. The nitrogen-fixing anabaena is creatively and simply treated to extract the extract liquid and the residues, and the extract liquid is concentrated and made into dry powders, the dry powders are made into the spray liquid to spray on the mulberry branches and leaves, and the residues are applied to the mulberry root soil. Yield ofmulberry leaves in each harvesting period is significantly increased during a growth period of mulberry trees, thereby significantly promoting the growth of mulberry trees.

Description

technical field [0001] The invention belongs to the field of agriculture, and in particular relates to the application of nitrogen-fixing anabaena to promoting the growth of mulberry trees and increasing the output of mulberry leaves. Background technique [0002] With the aggravation of water eutrophication, blue-green algae, mainly Microcystis sp., frequently break out in my country's large shallow lakes, urban ornamental and recreational lakes, and water supply reservoirs, causing great harm to my country's water ecological environment. . Salvage treatment is the most direct and effective measure to reduce the ecological disaster of cyanobacteria water pollution and reduce the intensity of the outbreak again. However, the landfill or long-term accumulation of salvaged cyanobacteria not only cannot effectively utilize the rich cyanobacteria organic matter, but also produces toxic substances such as algae toxins and polycyclic aromatic hydrocarbons, accompanied by harmful g...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G17/00A01C21/00
CPCA01C21/005A01G7/06A01G17/005
Inventor 卢梅雅
Owner 卢梅雅
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