Detection method for triptolide in tripterygium preparation

Abstract

The invention discloses and provides a detection method for triptolide in tripterygium preparation. The detection method comprises the following steps of (1) chromatographic condition, in which tadecyl silane chemically bonded silica is used as a filler, acetonitrile and water are used as a moving phase according to a volume proportion being 25:75, and the detection wavelength is 220 nanometers; 2) system applicability experiment, in which the theoretical plate number is calculated not to be lower than 2,000 according to the triptolide; 3) preparation of a contrast solution, in which a first methanol solution is added into the triptolide to prepare the contrast solution with triptolide having special concentration; 4) preparation of an experiment sample solution; and 5) measurement, in which a certain of the contrast solution obtained in the step 3) and the experiment sample solution obtained in the step 4) are absorbed and injected to a high performance liquid chromatograph for measurement, the column temperature is 30-40 DEG C, the flowing speed is 1.0ml / min, and the calculation is performed by an external standard method. By the detection method, the content of the triptolide inthe tripterygium preparation can be accurately detected, the clinical medication risk is judged in advance, and the clinical medication safety and effectiveness are ensured.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of the triptolide prepared from Huobahua root. Background technique [0002] The torch flower root is derived from the dry peeled root of Euonymus plant Kunming Mountain Begonia (Tripterygium hypoglaucum (Lev.) Hutch), tastes bitter, cold, and poisonous. Efficacy Expelling wind and dampness, relaxing tendons and activating collaterals, clearing away heat and detoxification. Clinically used for rheumatoid arthritis, rheumatoid arthritis, chronic nephritis, lupus erythematosus, bruises, itchy skin, etc., but it is toxic and should not be taken in excess. Modern pharmacological studies believe that it has anti-inflammatory, anti-immunosuppressive tumor effects and reproductive toxicity. [0003] The ingredients of preparations containing torch flower roots are relatively complex, mainly containing alkaloids, terpenes, lactones, and phenolic acids, among which triptolide is ...

AI Technical Summary

Problems solved by technology

[0003] The ingredients of preparations containing torch flower roots are relatively complex, mainly containing alkaloids, terpenes, lactones, and phenolic acids, among which triptolide is both an active ingredient and a toxic ingredient, which brings great risks to clinical medication

The detection of triptolide in the Huobahua root preparation mainly adopts steps such as ethanol reflux, adding acid to dissolve, extracting, separating, and evaporating to dryness, and the method of accurately weighing the residue to determine the total alkaloids. The repeatability is poor, and the content of triptolide cannot be accurately quantified

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