A mrfft2 cell

A cell and precise technology, applied in the field of MRFFT2 cells and its preparation, can solve the problems of self-protection technology of T cells, etc., and achieve the effect of strong target specificity, less off-target effect, and improved lethality

Active Publication Date: 2020-09-25
BEIJING DCTY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] None of the above schemes considers the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful tumor immune microenvironment

Method used

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  • A mrfft2 cell
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Embodiment Construction

[0058]The present invention is described below through specific embodiments. Unless otherwise specified, the technical means used in the present invention are methods known to those skilled in the art. In addition, the embodiments should be considered as illustrative rather than limiting the scope of the invention, the spirit and scope of which is defined only by the claims. For those skilled in the art, on the premise of not departing from the spirit and scope of the present invention, various changes or modifications to the material components and dosage in these embodiments also belong to the protection scope of the present invention.

[0059] 1. Epitope prediction

[0060] 1) Use the peripheral blood of lung cancer patients for ctDNA sequencing and HLA typing detection;

[0061] 2) Use software to analyze the sequencing information: compare the ctDNA sequencing results with the genome of normal cells, and screen out the mutation sites;

[0062] 3) Take the mutated amino...

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Abstract

The invention relates to an MRFFT2 cell and a preparation method thereof. Mutant polypeptides are screened by antigen epitope prediction, and are ligated to synthesize a mutant polypeptide expressiongene sequence; an MVA virus vector is constructed, an MVA virus is packaged, an APC cell is transferred, the transformation of a specific MV cell is completed, in vitro PBMC and a PBMC isolated from peripheral blood are co-cultured, an effective peptide is screened, an ordinary T cell is transformed through accurate and effective peptide stimulated secondary impact to form an RFF cell having an accurate killing capability, transformation is carried out by using a TCR-T technology principle, the modified T cell undergoes immunosuppressive signal closing by using antibody drug in vitro, so a specific killer T cell is accurately protected from being inhibited in vivo, and the lethality of the T cell on tumor cells is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a MRFFT2 cell and a preparation method thereof. Background technique [0002] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proved to be ineffective, while NK, CAR-NK, TIL and other cell technologies have yet to mature. CAR- T cells also have shortcomings in safety and solid tumor therapy. In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0003] The above treatment methods are immature, especially the in vitro induction of DC cells an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/863C12N15/90C12N15/867C12N5/10
CPCC07K14/7051C12N5/0636C12N15/86C12N15/907C12N2501/51C12N2501/998C12N2502/99C12N2510/00C12N2710/24143C12N2740/15043C12N2800/107C12N2810/10
Inventor 焦顺昌张嵘宋玉洁周子珊解佳森袁翰李营营
Owner BEIJING DCTY BIOTECH CO LTD
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