A filter device that prevents cell rupture

A filter device and cell technology, applied in the field of filtration, can solve problems such as whether the trapped cells are broken or not, and the analysis of cells that cannot be trapped, etc., to achieve the effect of convenient operation and streamlined overall structure

Active Publication Date: 2021-09-28
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition, there are other filtering devices. The purpose of these filtering devices is to filter and separate the target cells from the blood, without considering whether the trapped cells are broken or not, and cannot further filter the trapped cells. Analysis and other processing

Method used

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  • A filter device that prevents cell rupture
  • A filter device that prevents cell rupture
  • A filter device that prevents cell rupture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A filter device that prevents cell rupture, see figure 1 , including a filter unit, the filter unit includes a filter 1, a sample injection tube 2 and a liquid outlet tube 3, the details are as follows:

[0051] The filter 1 includes a body 1.1, a filter membrane 1.2, a sample inlet 1.3 and a liquid outlet 1.4, and the filter membrane 1.2 separates the cavity inside the body 1.1 into a first chamber and a second chamber that are independently arranged. chamber, the first chamber communicates with the injection tube 2 through the inlet 1.3, and the second chamber communicates with the outlet pipe 3 through the liquid outlet 1.4; the samples in the first chamber need to pass through All or part of the filter membrane 1.2 enters the second receiving chamber. The sampling tube 2 can also be provided with a valve 4 as required to control whether the sample passes through.

[0052] Here, the body 1.1 includes a base 1.11 and a cover 1.12, the base 1.11, the cover 1.12 and t...

Embodiment 2

[0059] A filter device that prevents cell rupture and can be used under a microscope, see image 3 , including a filter unit, the filter unit includes a filter 1, a sample injection tube 2 and a liquid outlet tube 3, the details are as follows:

[0060] The filter 1 includes a body 1.1, a filter membrane 1.2, a sample inlet 1.3 and a liquid outlet 1.4, and the filter membrane 1.2 separates the cavity inside the body 1.1 into a first chamber and a second chamber that are independently arranged. chamber, the first chamber communicates with the injection tube 2 through the inlet 1.3, and the second chamber communicates with the outlet pipe 3 through the liquid outlet 1.4; the samples in the first chamber need to pass through All or part of the filter membrane 1.2 enters the second receiving chamber.

[0061] The liquid outlet pipe 3 is a combination of a straight pipe and an arc pipe (arc pipe+straight pipe+arc pipe+straight pipe, connected in sequence), and the first end D1 of ...

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Abstract

The invention provides a filter device capable of preventing cell rupture, which includes a filter unit, the filter unit includes a filter, a sample inlet tube and a liquid outlet tube, and the filter includes a body, a filter membrane, a sample inlet and a liquid outlet. The filter membrane separates the cavity inside the body into a first chamber and a second chamber that are independently set, the first chamber communicates with the injection tube through the inlet, and the second chamber communicates with the injection tube through the outlet The liquid port is connected with the liquid outlet pipe; all the samples in the first chamber need to pass through the filter membrane to enter the second chamber in whole or in part; the outlet position of the liquid outlet pipe is not lower than 10mm below the filter membrane. Location. Applying the filtering device of the present invention, the effect is: the overall structure is simplified, and the operation is convenient; the position design of the outlet of the liquid outlet pipe is very particular, ensuring that the living cells trapped on the filter membrane do not directly contact the air, so that the trapped living cells remain activity, which is convenient for further research and analysis.

Description

technical field [0001] The invention relates to the technical field of filtration, in particular to a filtration device capable of preventing cell rupture. Background technique [0002] Cell filtration technology is widely used in the field of biomedicine. [0003] In the practice of scientific research, it is found that the fragmentation of the cell membrane is very easy to occur when capturing specific living cells for filtration, which leads to the destruction of the entire cell, which has a great impact on further analysis after filtration: on the one hand, after the cell ruptures, the intracellular fluid flows out , lead to cell death, the cells cannot maintain activity, resulting in extremely low efficiency of filtering and separating living cells; on the other hand, after cell rupture, various component proteins in the cell are lost (antigen loss), and various immunoassays are performed on the cells after filtration and analysis had significant adverse effects. [0...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12M1/12C12M1/00
CPCC12M23/02C12M23/22C12M23/38C12M23/48C12M29/04
Inventor喻风雷王理胡琪康刘文亮
OwnerTHE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV