Microbial activity color development enhancement method for petroleum corrosivity microbial detection kit
A technology for microbial detection and microbial activity, applied in the field of microbial detection, can solve the problems of medium becoming sour, accumulating acidic metabolites, and reducing the color of reduced products, etc., to achieve the effect of increasing sensitivity and enhancing color
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[0025] Example 1
[0026] This implementation case shows a method for enhancing the color development of microbial activity for the oil corrosive microorganism detection toolbox according to the following steps:
[0027] The microorganisms are grown on a solid medium containing MTT with a pH of 5.0 to 6.5 for three days to produce a reduction product of MTT. The alkaline solution used for spraying is a deionized aqueous solution of 10g / L sodium hydroxide with a pH value greater than 11. The alkaline solution is stored in a clean plastic spray can about 100mL. The plastic watering can can spray aerosol droplets scattered on the surface of the solid medium, spraying twice, each lasting about 1 second, and the spraying volume is 2 to 5 mL. After standing for 3 to 5 minutes, observe the color development on the medium.
Example Embodiment
[0028] Example 2
[0029] This implementation case shows a method for enhancing the color development of microbial activity for the oil corrosive microorganism detection toolbox according to the following steps:
[0030] The microorganisms are grown on a solid medium containing MTT with a pH of 4.0-5.5 for three days to produce a reduction product of MTT. The alkaline solution used for spraying is a distilled water solution of 20g / L sodium carbonate with a pH value greater than 12. The alkaline solution is stored in a clean plastic spray can about 100mL. The plastic watering can can spray aerosol droplets scattered on the surface of the solid medium, spraying twice, each lasting about 1 second, and the spraying volume is 2 to 5 mL. After standing for 1 to 5 minutes, observe the color development on the medium.
Example Embodiment
[0031] Example 3
[0032] This implementation case shows a method for enhancing the color development of microbial activity for the oil corrosive microorganism detection toolbox according to the following steps:
[0033] The microorganisms are grown on a solid medium containing MTT with a pH of 5.0 to 7.0 for three days to produce a reduction product of MTT. The alkaline solution used for spraying is a distilled water solution of 50g / L sodium bicarbonate with a pH value greater than 12. The alkaline solution is stored in a clean plastic spray can of about 250mL. The plastic watering can can spray aerosol-like droplets on the surface of the solid medium, spraying twice, each lasting about 2 seconds, and the spraying volume is 2 to 5 mL. After standing for 10-15 minutes, observe the color development on the medium.
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