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Method for establishing HPLC fingerprint of Zhongjiang peony and standard fingerprint thereof

A standard fingerprint, fingerprint technology, applied in the establishment method of Zhongjiang Paeonia lactiflora HPLC fingerprint and the field of standard fingerprint, can solve the problem of not being able to comprehensively evaluate the quality of medicinal materials, and achieve the effect of extensive system adaptability

Inactive Publication Date: 2019-03-29
SICHUAN AGRI UNIV
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Problems solved by technology

"The Pharmacopoeia of the People's Republic of China" (2015 Edition, Part One) stipulates that the content of paeoniflorin (not less than 1.6% and 1.8%) in Radix Paeoniae Alba and Radix Paeoniae Rubra should be used to control the quality of Radix Paeoniae Alba and Radix Radix Radix Paeoniae Alba, but this single index The quality control of ingredients cannot comprehensively evaluate the quality of medicinal materials

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  • Method for establishing HPLC fingerprint of Zhongjiang peony and standard fingerprint thereof
  • Method for establishing HPLC fingerprint of Zhongjiang peony and standard fingerprint thereof
  • Method for establishing HPLC fingerprint of Zhongjiang peony and standard fingerprint thereof

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Embodiment Construction

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0022] see Figure 1-10, the present invention provides a technical solution: a method for establishing the HPLC fingerprint of Chinese Paeoniae Alba and its standard fingerprint, the steps are as follows; a, the selection of reagents and instruments; the selection of reagents and instruments in the step a is a standard product: Paeoniflorin, paeoniflorin, hydroxypaeoniflorin, benzoylpaeoniflorin, gallic acid, catechin, purity > 98%, methanol (HPLC grade), ace...

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Abstract

The invention relates to a method for establishing HPLC fingerprint of Zhongjiang peony and a standard fingerprint thereof. The method comprises the following steps of: a, selecting reagents and instruments; b, preparing a Zhongjiang peony sample solution; c, preparing a reference solution; d, precisely sucking the sample solution for a HPLC analysis, separately; and e, exporting the fingerprint data of the Zhongjiang peony obtained in step d, introducing a chromatographic fingerprint similarity evaluation system of the traditional Chinese medicine, selecting the chromatographic peaks existingin the chromatograms of different batches of the Zhongjiang peony as a common peak, and calculating the relative retention time and the relative peak area of the common peak. The method for detectingHPLC fingerprint of the Zhongjiang peony provided by the invention has the advantages of simple method, stability, high precision and good reproducibility, and can comprehensively and objectively characterize the quality of the Zhongjiang peony.

Description

technical field [0001] The invention relates to the technical field of processing Paeoniae Zhongjiang, in particular to a method for establishing an HPLC fingerprint of Paeoniae Zhongjiang and a standard fingerprint thereof. Background technique [0002] Fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum that can mark the chemical characteristics of some traditional Chinese medicinal materials or preparations after proper treatment and certain analysis methods. Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and semi-finished products of traditional Chinese medicine preparations. " "Integrity" and "ambiguity" are its salient features. At present, FDA, WHO, British Herbal Codex,...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86G01N30/04G01N30/06G01N30/14G01N30/74G01N30/34
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/74G01N30/8634G01N30/8686G01N2030/042
Inventor 张利吴一超姜媛媛李杨梅张绍奇王龙余燕周劲
Owner SICHUAN AGRI UNIV
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