Method for establishing HPLC fingerprint of Zhongjiang peony and standard fingerprint thereof

Abstract

The invention relates to a method for establishing HPLC fingerprint of Zhongjiang peony and a standard fingerprint thereof. The method comprises the following steps of: a, selecting reagents and instruments; b, preparing a Zhongjiang peony sample solution; c, preparing a reference solution; d, precisely sucking the sample solution for a HPLC analysis, separately; and e, exporting the fingerprint data of the Zhongjiang peony obtained in step d, introducing a chromatographic fingerprint similarity evaluation system of the traditional Chinese medicine, selecting the chromatographic peaks existingin the chromatograms of different batches of the Zhongjiang peony as a common peak, and calculating the relative retention time and the relative peak area of the common peak. The method for detectingHPLC fingerprint of the Zhongjiang peony provided by the invention has the advantages of simple method, stability, high precision and good reproducibility, and can comprehensively and objectively characterize the quality of the Zhongjiang peony.

Description

technical field [0001] The invention relates to the technical field of processing Paeoniae Zhongjiang, in particular to a method for establishing an HPLC fingerprint of Paeoniae Zhongjiang and a standard fingerprint thereof. Background technique [0002] Fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum that can mark the chemical characteristics of some traditional Chinese medicinal materials or preparations after proper treatment and certain analysis methods. Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and semi-finished products of traditional Chinese medicine preparations. " "Integrity" and "ambiguity" are its salient features. At present, FDA, WHO, British Herbal Codex,...

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Problems solved by technology

"The Pharmacopoeia of the People's Republic of China" (2015 Edition, Part One) stipulates that the content of paeoniflorin (not less than 1.6% and 1.8%) in Radix Paeoniae Alba and Radix Paeoniae Rubra should be used to control the quality of Radix Paeoniae Alba and Radix Radix Radix Paeoniae Alba, but this single index The quality control of ingredients cannot comprehensively evaluate the quality of medicinal materials

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