Mesenchymal stem cell (MSC) isolated culture device and isolating method thereof

Abstract

The invention discloses a mesenchymal stem cell (MSC) isolated culture device and an isolating method thereof. The MSC isolated culture device comprises a culture sleeve, culture dishes, handles, culture mediums, suspension rods, circular pipes, droppers, supporting seats, a first connector, a sealing cover, a liquid storing tank, conducting wires, second connectors, dustproof sleeves, a guiding-in pipe, a guiding-out pipe, a switch, a junction box, a transfer pump, a motor, a motor switch, supporting frames, a supporting block, an arc-shaped bayonet, a rotating column, a fixed block, a dustproof plug, a separating pipe, a funnel opening, a long pipe, a storing chamber, a third connector, a supporting cylinder, a fixed bayonet, a filter screen and a fourth connector. According to the MSC isolated culture device disclosed by the invention, liquid to be isolated in the liquid storing tank is sucked by the transfer pump through the guiding-in pipe, is guided into the separating pipe through the fourth connector by the guiding-out pipe, after isolation, filtration is performed with a filter screen, and required MSC liquid is reserved; the required MSC liquid is guided into the circularpipe through the third connector by the duct; the MSC liquid flows out from the droppers to the culture mediums, so that accidents including infection and the like in the transporting process are avoided; and a plurality of cavities are formed in the culture sleeve, so that the culture mediums can be accommodated.

Description

technical field [0001] The invention relates to a culture device and a separation method thereof, in particular to a MSC separation culture device and a separation method thereof, belonging to the technical field of separation culture applications. Background technique [0002] Mesenchymal stem cells (MSCs) are an important classification of stem cells, derived from the mesoderm in the early stage of development, belonging to pluripotent stem cells, with the characteristics of multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. The clinical application of mesenchymal stem cells to solve a variety of blood system diseases, cardiovascular diseases, liver cirrhosis, nervous system diseases, partial knee meniscectomy injury repair, autoimmune diseases, etc. have made major breakthroughs and saved more patients. suffering life. In addition, mesenchymal stem cells have long-term developmen...

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Problems solved by technology

[0003] However, during the use of the traditional MSC isolation and culture device and its isolation method, it is easy to happen that the whole medium is unusable due to infection of part of the medium, and the separated MSC liquid needs to be transferred to the medium again. In the process of transportation, it may cause accidents such as infection

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