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Research method for repairing skin by preparing stem cell lyophilized powder

A technology of freeze-dried powder and stem cells, which is applied in the field of stem cell applications and can solve problems such as lack of universal applicability, high price, and immune rejection

Pending Publication Date: 2020-07-07
HENAN YINFENG BIOENG CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, the application of stem cells in anti-aging cosmetics has also become a research hotspot. However, there are some so-called stem cell injections in the beauty industry on the market. Injection of embryonic stem cells directly, which is easy to cause immune rejection, costs a lot, and the price is high. It needs to be customized according to individual conditions, and there is no universal applicability.

Method used

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  • Research method for repairing skin by preparing stem cell lyophilized powder

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Embodiment Construction

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0025] see figure 1 , a research method for skin repair by preparing dry cell freeze-dried powder, comprising the following steps:

[0026] Step 1, extracting mesenchymal stem cells, and using the adult mesenchymal stem cells extracted from blood for serum-free culture;

[0027] Step 2: Cultivate the mesenchymal stem cells, put the extracted mesenchymal stem cells into a serum-free medium for cultivation, and add serum serotonin, L-g...

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Abstract

The invention discloses a research method for repairing skin by preparing stem cell lyophilized powder. The method includes the steps of extracting mesenchymal stem cells, culturing the mesenchymal stem cells, preparing the stem cell lyophilized powder, performing a skin wound healing experiment, and performing data statistical analysis. The method has the following beneficial effect: when the mesenchymal stem cells are extracted, a liquid to be separated in a liquid storage tank is sucked out by using a delivery pump through an introduction tube, a required MSC liquid is left after filtrationthrough a filter net and flows out to a culture medium, a culture sleeve is provided with multiple hollow cavities for holding the culture medium, so that accidents such as infection during transportation are avoided, and the problem of mutual infection is also avoided; the oxygen concentration of the mesenchymal stem cell culture is 2-3%, the temperature is 37 DEG C, the carbon dioxide concentration is 5%, under the low oxygen concentration, the expansion of adult stem cells is promoted, and the cycle of cell multiplication is shortened; and the lyophilized powder of the adult stem cells contains polypeptide substances such as an epidermal growth factor and a fibroblast growth factor, and can repair damaged cells and promote the growth and growth of skin cells.

Description

technical field [0001] The invention relates to a research method for dry cell freeze-dried powder, in particular to a research method for skin repair by preparing dry cell freeze-dried powder, and belongs to the technical field of stem cell application. Background technique [0002] Stem cells are a type of cells with self-replication ability and multi-directional differentiation potential. Under certain conditions, they can differentiate into a variety of functional cells and tissues. They also have the biological functions of immune regulation, repair, and regeneration of damaged tissues and aging organs. Function. Studies have found that stem cells have high telomerase activity, which is the youthful vitality factor of the human body. development occurs. [0003] Skin aging refers to the aging damage of skin function, which reduces the skin's ability to protect and regulate the body. Skin aging is manifested in small wrinkles, decreased elasticity, and loose skin. Wit...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K49/00
CPCC12N5/0662A61K49/0008C12N2509/00C12N2500/32C12N2500/40C12N2501/115C12N2501/15
Inventor 张雪梅程志远王小龙王彦陈乐
Owner HENAN YINFENG BIOENG CO LTD
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