Preparation method of stem cell freeze-dried powder based on cell culture

Abstract

The invention discloses a preparation method of stem cell freeze-dried powder based on cell culture, which comprises the steps of extracting mesenchymal stem cells, culturing the mesenchymal stem cells, collecting supernate of a culture solution, filtering, concentrating, freezing and drying. The preparation method has the beneficial effects that when mesenchymal stem cells are extracted, to-be-separated liquid in a liquid storage tank is sucked out by a delivery pump through an ingress pipe, the required MSC liquid is filtered by a filter screen and flows out to a culture medium, and a culture sleeve is provided with a plurality of cavities which can be used for accommodating the culture medium, so that accidents such as infection in the transportation process are avoided, meanwhile, the problem of mutual infection is also avoided. The oxygen concentration of mesenchymal stem cell culture is 2-3%, the temperature is 37 DEG C, and the carbon dioxide concentration is 5%, under low oxygen concentration, the expansion of adult stem cells is promoted, and the cell growth cycle is shortened; a freeze-dried powder protective agent is used, and then the freeze-dried powder is filtered and sterilized through a 0.21[mu]m filter membrane, so that the high purity of the prepared freeze-dried powder is ensured, and the repair function is enhanced.

Description

technical field [0001] The invention relates to a preparation method of stem cell freeze-dried powder, in particular to a preparation method of stem cell freeze-dried powder based on cell culture, and belongs to the technical field of freeze-dried powder preparation. Background technique [0002] Stem cells are a type of cells with self-replication ability and multi-directional differentiation potential. Under certain conditions, they can differentiate into various functional cells and tissues. They also have the biological functions of immune regulation, repair, and regeneration of damaged tissues and aging organs. Features. Studies have found that stem cells have high telomerase activity, which is the youthful vitality factor of the human body. With the growth of age, the number of stem cells in the human body will continue to decrease, and the potential for proliferation and differentiation will also continue to weaken, directly leading to aging and disease. Development ...

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Problems solved by technology

[0004] In recent years, the application of stem cells in anti-aging cosmetics has also become a research hotspot. However, there are some so-called stem cell injections in the beauty industry on the market. Inject embryonic stem cells directly, which is likely to cause immune rejection, cost a lot, and the price is high. It needs to be customized according to the individual situation. There is no universal applicability, and the cost is high in the process of preservation and preparation.

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