Method for researching skin whitening effect by adopting stem cell exosome

Abstract

The invention discloses a method for researching a skin whitening effect by adopting stem cell exosome. The method comprises the following steps: extracting mesenchymal stem cells, culturing the mesenchymal stem cells, preparing stem cell exosome freeze-dried powder, carrying out a skin whitening effect experiment and carrying out data statistical analysis. The method has the beneficial effects that: a conveying pump is used for sucking out a to-be-separated liquid in a liquid storage tank through an ingress pipe, the to-be-separated liquid is filtered through a filter screen, required MSC liquid is left and flows out to a culture medium, a plurality of cavities are formed in a culture sleeve and can be used for containing the culture medium, accidents such as infection in the transportation process are avoided, and meanwhile, the problem of mutual infection is also avoided; and wherein the oxygen concentration of the mesenchymal stem cell culture is 2-3%, the temperature is 37C, the carbon dioxide concentration is 5%, and under the low oxygen concentration, the expansion of the adult stem cells is promoted, and the cell multiplication cycle is shortened.

Description

technical field [0001] The invention relates to a method for researching stem cell exosomes, in particular to a research method for skin whitening effect using stem cell exosomes, and belongs to the technical field of stem cell applications. Background technique [0002] Exosomes are nanoscale lipid bilayer vesicles released by exocytosis to the outside of cells, and can exist in various types of extracellular body fluids, such as blood, urine, amniotic fluid, saliva, cerebrospinal fluid, and even breast milk. It is found that exosomes contain cell-specific proteins, lipids and nucleic acids, which can be transmitted to other cells as signaling molecules, thereby changing the functions of other cells, such as: exosomes participate in neuron communication, antigen presentation, immune response, organ Regulates fundamental physiological processes such as development and reproduction, and is also involved in the regulation of pathological processes such as cancer progression, c...

AI Technical Summary

Problems solved by technology

[0004] In recent years, the application of stem cells in whitening cosmetics has also become a research hotspot. However, there are some so-called stem cell injections in the beauty industry on the market. The factors secreted by stem cells can provide various nutritional and material information needed for cell growth and differentiation, and can quickly activate the body's repair system, and carry out intelligent repair according to different skin problems

Images