Research method for skin tightening effect by adopting stem cell exosomes

Abstract

The invention discloses a research method for a skin tightening effect by adopting stem cell exosomes. The research method comprises the following steps: extracting mesenchymal stem cells, cultivatingthe mesenchymal stem cells, preparing stem cell exosome freeze-dried powder, performing a skin tightening effect experiment, and performing data statistic analysis. When the mesenchymal stem cells are extracted, to-be-separated liquid in a liquid storage tank is sucked out by utilizing a delivery pump through a guide-in pipe, the to-be-separated liquid is filtered by virtue of a filter screen toleave required MSC liquid, the MSC liquid flows out to a culture medium, and a culture sleeve is provided with a plurality of cavities which can be used for accommodating the culture medium, so that accidents such as infection in a transportation process can be avoided, and meanwhile, the problem of mutual infection can be avoided; and the oxygen concentration of mesenchymal stem cell culture is 2-3%, the temperature is 37 DEG C, the carbon dioxide concentration is 5%, amplification of the adult stem cells can be promoted under the low oxygen concentration, and the cell multiplication period can be shortened.

Description

technical field [0001] The invention relates to a method for researching stem cell exosomes, in particular to a method for researching the effect of stem cell exosomes on skin firming, and belongs to the technical field of stem cell applications. Background technique [0002] Exosomes are nanoscale lipid bilayer vesicles released by exocytosis to the outside of cells, and can exist in various types of extracellular body fluids, such as blood, urine, amniotic fluid, saliva, cerebrospinal fluid, and even breast milk. It is found that exosomes contain cell-specific proteins, lipids and nucleic acids, which can be transmitted to other cells as signaling molecules, thereby changing the functions of other cells, such as: exosomes participate in neuron communication, antigen presentation, immune response, organ Regulates fundamental physiological processes such as development and reproduction, and is also involved in the regulation of pathological processes such as cancer progressi...

AI Technical Summary

Problems solved by technology

[0004] In recent years, the application of stem cells in skin-tightening cosmetics has also become a research hotspot. However, there are some so-called stem cell injections in the beauty industry on the market. Most of the active ingredients are single, and the effect on maintaining skin firmness is not Very good, there are many products on the market to keep the skin tight, such as jasmine essential oil, rosemary essential oil, etc. The market is broad, but many products will cause rebound when not used for a long time

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